Diosmetin (C16H12O6; Fig. 1A ) was purchased from Sigma-Aldrich (St. Louis, MO, USA). The original concentration of Dio stored at −20°C was 10 mg/ml. TGF-β human recombinant was purchased from Prospec-Tany TechnoGene, Ltd. (East Brunswick, NJ, USA) and anti-human antibodies against p53, Bcl-2, Bax, TGF-β, TβRII, Smad3, phosphorylated (p)-Smad2/3 and GADPH were all purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). Horseradish peroxidase (HRP)-conjugated goat anti-rabbit immunoglobulin G (IgG) secondary antibody was obtained from EarthOx Life Sciences, LLC (Millbrae, CA, USA). Cell Counting Kit-8 (CCK8) and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) were purchased from Beyotime Institute of Biotechnology (Haimen, China).
3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2 h tetrazolium bromide mtt
Manufactured by Beyotime
Sourced in China
3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) is a chemical compound used as a colorimetric assay for assessing cell metabolic activity. It is a pale yellow tetrazolium salt that is reduced to a dark blue formazan product by the action of dehydrogenase enzymes found in metabolically active cells.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Ethanol, by Xilong (3 mentions)
Petroleum ether, by Xilong (3 mentions)
Ethyl acetate, by Xilong (3 mentions)
N butanol, by Xilong (3 mentions)
Ripa lysis buffer, by Ipsen (2 mentions)
Coomassie blue staining solution, by Beyotime (2 mentions)
Reactive oxygen species assay kit, by Yeasen (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Tβrii, by Cell Signaling Technology (1 mentions)
Tgf β, by Cell Signaling Technology (1 mentions)
P smad2 3, by Cell Signaling Technology (1 mentions)
Cell counting kit 8 cck 8, by Beyotime (1 mentions)
Protein ladder, by Thermo Fisher Scientific (1 mentions)
Annexin 5 fitc pi apoptosis detection kit, by Beyotime (1 mentions)
Penicillin streptomycin 100, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Sinopharm (1 mentions)
Cellulose dialysis membrane, by Merck Group (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
9 protocols using 3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2 h tetrazolium bromide mtt
1
Diosmetin Modulates TGF-β Signaling
2
Cytotoxicity Assay Protocol for CDDP and OLA
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All reagents used in this study were commercially available and did not undergo any additional purification. CDDP and OLA were obtained from Meilun Biotech (Dalian, China). Dimethyl sulfoxide (DMSO) was supplied by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Phosphate Buffered Saline (PBS), Dulbecco’s Modified Eagle’s Medium (DMEM), Fetal Bovine Serum (FBS), 0.25% pancreatin, and penicillin-streptomycin (100×) were acquired from Gibco (Grand Island, USA). 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), Annexin V-FITC/PI apoptosis detection kits, cell cycle test kits, and mitochondrial membrane potential (MMP) detection kits were obtained from Beyotime Biotechnology Co., LTD. (Shanghai, China). Hoechst live cell stain was acquired from Sigma Aldrich Co. (St Louis, MO, USA). All antibodies used for Western blotting were purchased from Affinity Biosciences (Jiangsu, China), and the protein ladder was obtained from ThermoFisher Scientific (Waltham, MA, USA). Cell experiment consumables were sourced from Corning Incorporated (Corning, NY, USA).
3
Optimized Silk Fibroin for Cell Culture
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Bombyx mori raw silk fibers were purchased from Soho Biotechnology Co. Ltd. (China). PVA (MW ∼31 000, 88% hydrolysed), absolute ethanol, calcium chloride (CaCl2), sodium carbonate (Na2CO3), horseradish peroxidase (HRP), hydrogen peroxide (H2O2), and paeonol were purchased from Shanghai Aladdin Bio-Chem Technology Co., LTD (China). Cellulose dialysis membranes (molecular weight cut-off of 12 kDa) were purchased from Sigma-Aldrich (USA). Roswell Park Memorial Institute medium 1640 (RPMI-1640, Gibco), penicillin/streptomycin (HyClone), trypsin (HyClone) and fetal bovine serum (FBS, Gibco) were purchased from Shanghai Pufei Bio-Technology Co. Ltd. (China). Apoptosis and necrosis assay kit (Hoechst 33342 and PI), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), and dimethyl sulfoxide (DMSO) were purchased from Beyotime Institute of Biotechnology (China). L929 cell line (ATCC CCL-1) were purchased from American Type Culture Collection (ATCC, USA). All other chemicals were of analytical grade, purchased from Shanghai Sinopharm Chemical Reagent Co. Ltd. (China) and used without further purification.
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Bombyx mori raw silk fibers were purchased from Soho Biotechnology Co. Ltd. (China). PVA (MW ∼31 000, 88% hydrolysed), absolute ethanol, calcium chloride (CaCl2), sodium carbonate (Na2CO3), horseradish peroxidase (HRP), hydrogen peroxide (H2O2), and paeonol were purchased from Shanghai Aladdin Bio-Chem Technology Co., LTD (China). Cellulose dialysis membranes (molecular weight cut-off of 12 kDa) were purchased from Sigma-Aldrich (USA). Roswell Park Memorial Institute medium 1640 (RPMI-1640, Gibco), penicillin/streptomycin (HyClone), trypsin (HyClone) and fetal bovine serum (FBS, Gibco) were purchased from Shanghai Pufei Bio-Technology Co. Ltd. (China). Apoptosis and necrosis assay kit (Hoechst 33342 and PI), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), and dimethyl sulfoxide (DMSO) were purchased from Beyotime Institute of Biotechnology (China). L929 cell line (ATCC CCL-1) were purchased from American Type Culture Collection (ATCC, USA). All other chemicals were of analytical grade, purchased from Shanghai Sinopharm Chemical Reagent Co. Ltd. (China) and used without further purification.
4
Paclitaxel-Coumarin-6 Nanoparticle Evaluation
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Paclitaxel (≥99.0%) and coumarin-6 (≥98.0%) were obtained from Shanghai Aladdin Biochemical Technology, China. Triptolide (≥98.0%) was purchased from Nantong Feiyu Biological Technology, China. Polyvinyl alcohol (PVA, MW 15 000 kDa; hydrolyzed 87%–89%) was purchased from Energy Chemical, China. RIPA lysis buffer and sodium dodecyl sulfate-polyacrylamide gel were obtained from Epizyme, China. Coomassie Brilliant Blue G-250, BCA protein assay kit, Coomassie Blue Staining Solution, antifade mounting medium with DAPI, and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) were purchased from Beyotime, China. Dimethyl sulfoxide (DMSO) was purchased from Sigma-Aldrich, China. Phosphate buffered saline (PBS) solution was obtained from Cytiva, USA. Transwell plates were obtained from Corning Costar, USA. Crystalline violet staining solution and 4% paraformaldehyde were purchased from Solarbio, China.
5
Annexin V-FITC Apoptosis Assay
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The Annexin V-FITC apoptosis detection kit was purchased from Nanjing KeyGen Biotech (Nanjing, China). 3-(4,5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT) was obtained from Beyotime (Beyotime Institute of Biotechnology, Shanghai, China). Dulbecco's modified Eagle's medium was purchased from Hyclone (GE Healthcare, Logan, UK).
6
Resveratrol and Coumarin-6 Synergistic Nanoformulation
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Resveratrol (RSV, ≥98.0%) and Coumarin-6 (C6, ≥98.0%) were purchased from Aladdin Biochemical Technology (Shanghai, China). mPEG5K-PCL20K was got from Ruixi (Xi'an, China). RIPA lysis buffer and skimmed milk powder were got from Epizyme (Shanghai, China). Malondialdehyde (MDA) assay, SLC7A11/xCT Rabbit Polyclonal Antibody, GPX4 Rabbit Polyclonal Antibody, and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) were obtained from Beyotime (Nanjing, China). Reactive oxygen species assay kit and JC-1 mitochondrial membrane potential assay kit were got from Yeasen Biotechnology (Shanghai, China). DAPI was obtained from Fcmacs Biotech (Nanjing, China). RPMI1640 and Fetal bovine serum (FBS) were got from Biological Industries (Israel).
7
Ghrelin's Impact on Cellular Oxidative Stress
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Ghrelin was purchased from Sigma Chemical (St. Louis, MO, USA) and dissolved in dimethylsulfoxide (DMSO) (Sigma, St. Louis, MO). Fetal bovine serum (FBS) and Dulbecco's modified Eagle's medium (DMEM) were obtained from Gibco (Grand Island, NY, USA). Medium 199 was obtained from Sigma-Aldrich (St. Louis, MO). Annexin V-FITC and propidium iodide (PI) were obtained from Becton Dickinson (Mountain View, CA, USA). 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) and H2DCFDA were obtained from Beyotime (Beyotime Institute of Biotechnology, Shanghai, China). Anti-SOD, anti-CAT, and anti-MDA antibodies were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). SOD, CAT, and MDA colorimetric kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).
8
Extraction and Evaluation of Active Compounds from AGS
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The roots and rhizomes of AGS were purchased from Guangzhou Zhixin medicinal herbs Co., Ltd (Lot number: 20,191,201, Guangdong, China) and identified by Professor Suying Tian (Guangdong Pharmaceutical University, Guangzhou, China). The ethanol, petroleum ether (30–60 ℃, 60–90 ℃), ethyl acetate, and n-butanol were obtained from Xilong Chemical Co., Ltd (Guangdong, China). Diatomite was obtained from Dingshengxin Chemical Co., Ltd (Tianjin, China). McCoy’s 5 A medium was purchased from Procell Life Science&Technology Co., Ltd (Wuhan, China). Leibovitz’s L-15 medium was purchased from Dalian Meilun Biotechnology Co., Ltd (Dalian, China). Fetal bovine serum, phosphate-buffered saline, 5% trypsin-EDTA were purchased from Gibco (Carlsbad, CA, United States). Penicillin-streptomycin (P/S) and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) were purchased from Beyotime Biotechnology (Shanghai, China).
9
Ursolic Acid and Coumarin-6 Nanoformulation
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Ursolic acid (≥99.0%) and coumarin-6 (≥98.0%) were purchased from Macline Biochemical Technology Co., Ltd. (Shanghai, China). Poloxamer 188 PRO was purchased from Sigma-Aldrich (Shanghai, China). 1,1′-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (Dil), BCA protein assay kit, Coomassie blue staining solution, and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) were all purchased from Beyotime Biotechnology Co., Ltd. (Nantong, China). Crystalline violet staining solution, 4% paraformaldehyde, APC annexin V apoptosis detection kit, and reactive oxygen species assay kit were purchased from Yeasen (Shanghai, China).
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