Three confluent 160-mm-diameter dishes of MBECs were harvested and plasma membranes were isolated as previously described (Yao et al., 2009). Protein concentrations were determined by Bio-Rad Dc-Protein assay (Bio-Rad). Samples were mixed with Bio-Rad XT sample buffer and reducing agent, run on Criterion™ XT 4–12% Bis-Tris Gels (Bio-Rad), and transferred onto PVDF membranes. Immunodetection was performed with (1:100) rabbit anti-connexin 43 antibody (Invitrogen), (1:500) mouse anti phospho-caveolin1 (pY14) (BD Transduction Laboratories), (1:5000) rabbit anti-β-catenin (Abcam), (1:1000) rabbit anti-Cav-1 (Abcam), or (1:1000) mouse-anti-transferrin receptor (Life Technology) followed by HRP-coupled secondary antibodies (Jackson ImmunoResearch), and developed with ECL-Plus (GE Healthcare). Protein bands were quantified by densitometry using Image Lab 5.1 (Bio-Rad) software. Plasma membrane connexin43 levels were normalized to plasma membrane transferrin receptor. This value was normalized to total β-catenin as a housekeeping reference protein.
Mouse anti phospho caveolin1 py14
Manufactured by BD
The mouse anti phospho-caveolin1 (pY14) is a primary antibody that detects caveolin-1 phosphorylated at tyrosine 14. It can be used for the detection and analysis of phosphorylated caveolin-1 in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Hrp coupled secondary antibody, by Jackson ImmunoResearch (2 mentions)
Image lab 5, by Bio-Rad (2 mentions)
Rabbit anti cav 1, by Abcam (2 mentions)
Mouse anti transferrin receptor, by Thermo Fisher Scientific (2 mentions)
Criterion xt 4 12 bis tris gel, by Bio-Rad (2 mentions)
Rabbit anti connexin 43 antibody, by Thermo Fisher Scientific (2 mentions)
Dc protein assay, by Bio-Rad (2 mentions)
Rabbit anti β catenin, by Abcam (2 mentions)
Ecl plus, by GE Healthcare (2 mentions)
2 protocols using mouse anti phospho caveolin1 py14
1
Plasma Membrane Protein Analysis
2
Plasma Membrane Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Three confluent 160-mm-diameter dishes of MBECs were harvested and plasma membranes were isolated as previously described (Yao et al., 2009). Protein concentrations were determined by Bio-Rad Dc-Protein assay (Bio-Rad). Samples were mixed with Bio-Rad XT sample buffer and reducing agent, run on Criterion™ XT 4–12% Bis-Tris Gels (Bio-Rad), and transferred onto PVDF membranes. Immunodetection was performed with (1:100) rabbit anti-connexin 43 antibody (Invitrogen), (1:500) mouse anti phospho-caveolin1 (pY14) (BD Transduction Laboratories), (1:5000) rabbit anti-β-catenin (Abcam), (1:1000) rabbit anti-Cav-1 (Abcam), or (1:1000) mouse-anti-transferrin receptor (Life Technology) followed by HRP-coupled secondary antibodies (Jackson ImmunoResearch), and developed with ECL-Plus (GE Healthcare). Protein bands were quantified by densitometry using Image Lab 5.1 (Bio-Rad) software. Plasma membrane connexin43 levels were normalized to plasma membrane transferrin receptor. This value was normalized to total β-catenin as a housekeeping reference protein.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!