Ostrich lungs were prepared from Mashhad Meat Industrial Complex (Mashhad, Iran), and ACE from ostrich lung was purified as previously reported [14 ]. Fresh ostrich egg was prepared from Esteghlal market (Mashhad, Iran). Pepsin (porcine gastric mucosa, EC 3.4.23.1), pancreatin (porcine pancreas), 1,1-diphenyl-2-picrylhydrazyl (DPPH), glutathione (GSH), 2,20-azinobis(3-ethylbenzothiazoline-6-sulphonicacid) (ABTS), potassium persulfate, potassium phosphate, trichloroacetic acid (TCA), ferric chloride, 3-(4,5-dimethylthia-zol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), ethylenediaminetetra-acetic acid (EDTA), and trifluoroacetic acid (TFA) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Ultrafiltration membranes with a 3-kDa cut off were purchased from Millipore (Bedford, MA, USA).
Ultrafiltration membranes with a 3 kda cut off
Manufactured by Merck Group
Sourced in United States
Ultrafiltration membranes with a 3-kDa cut off are a type of filtration technology used for the separation and purification of molecules. These membranes have a molecular weight cut-off of 3 kilodaltons, allowing the passage of smaller molecules while retaining larger ones.
Automatically generated - may contain errors
Lab products found in correlation
Pepsin, by Merck Group (1 mentions)
Pancreatin, by Merck Group (1 mentions)
Ethylenediaminetetraacetic acid edta, by Merck Group (1 mentions)
2 20 azino bis 3 ethyl benzo thiazoline 6 sulphonic acid, by Merck Group (1 mentions)
1 1 diphenyl 2 picrylhydrazyl, by Merck Group (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Trichloroacetic acid tca, by Merck Group (1 mentions)
Potassium phosphate, by Merck Group (1 mentions)
Glutathione (gsh), by Merck Group (1 mentions)
Trifluoroacetic acid tfa, by Merck Group (1 mentions)
Potassium persulfate, by Merck Group (1 mentions)
Captopril, by Merck Group (1 mentions)
Fapgg, by Merck Group (1 mentions)
Trypsin, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
2 protocols using ultrafiltration membranes with a 3 kda cut off
1
Ostrich Lung ACE Purification and Antioxidant Analysis
2
ACE Inhibition Assay Protocol
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TFA (trifluoroacetic acid), acetonitrile, trypsin (from bovine pancreas), papain (from pawpaw sap), FAPGG (N-(3-[2-furylacryloyl-Phe-Gly-Gly])), ACE (angiotensin-I converting enzyme) from rabbit lung and captopril (≥98% purity), were purchased from Sigma-Aldrich Co. (Saint Louis, MO, USA). Ultrafiltration membranes with a 3 kDa cut-off were purchased from Millipore (Bedford, MA, USA). Analytical and semi-preparative columns were purchased from Macherey Nagel GmbH Co. (St. Neumann Neander, Düren, Germany). All other chemicals used were of analytical grades.
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