The following antibodies were used at specified dilutions for immunoblotting: polyclonal PARP1 antibody (1:5,000; Alexis Biochemicals), PARP1 monoclonal (F2, 1:500; Santacruz), PARP1 C-2-10 (1:1,000), PARP1 89kDa (1:1,000, Abcam), p53 (1:1,000, Boehringer), gH2AX (1:1,000, Millipore), phospho-p53 (1:2,000, NEB), p21 (1:1,000, NEB0), β-actin (1:20,000, Sigma), cleaved caspase-9 (1:1,000, Cell Signaling), cleaved caspase-3 (1:1,000, Cell Signaling), cleaved caspase-7 (1:200, Abcam), Ki-67 (1:100, Thermo scientific), PCNA (1:1,000, Neomarkers). Anti-pADPr monoclonal 10H was purified from the culture medium of 10H hybridoma obtained from Dr. M. Miwa, National Cancer Center Research Institute, Tokyo, through the Riken cell bank (1:500). The secondary HRP-conjugated antibodies (1:1,250) were purchased from Jackson Immunoresearch Laboratories.
Phospho p53
Manufactured by New England Biolabs
Sourced in United Kingdom
Phospho-p53 is a product that detects the phosphorylated form of the p53 protein, a key regulator of cellular responses to DNA damage and other cellular stresses. This product can be used to study the activation and regulation of the p53 signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
Gh2ax, by Merck Group (1 mentions)
Cleaved caspase 7, by Abcam (1 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Cleaved caspase 9, by Cell Signaling Technology (1 mentions)
β actin, by Merck Group (1 mentions)
Hrp conjugated antibodies, by Jackson ImmunoResearch (1 mentions)
Phospho akt, by New England Biolabs (1 mentions)
Hydrogen peroxide solution, by Merck Group (1 mentions)
Cleaved caspase 3 c caspase 3, by Santa Cruz Biotechnology (1 mentions)
Poly adp ribose polymerase 1 parp 1, by Santa Cruz Biotechnology (1 mentions)
Akt inhibitor, by Merck Group (1 mentions)
Lycopene, by Merck Group (1 mentions)
Bcl2 associated x (bax), by Santa Cruz Biotechnology (1 mentions)
Fetal bovine serum (fbs), by Lonza (1 mentions)
2 protocols using phospho p53
1
Antibody Immunoblotting Protocol
2
Oxidative Stress-Induced Signaling in hMSCs
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Human MSCs (hMSCs) were obtained from American Type Culture Collection (Manassas, VA, USA). Fetal bovine serum (FBS) was purchased from Biowhittaker (Walkersville, MD, USA). Hydrogen peroxide solution was obtained from the Sigma Chemical Company (St. Louis, MO, USA). Phospho-p38 mitogen-activated protein kinase (MAPK), p38 MAPK, phospho-c-Jun N-terminal kinase (JNK), JNK, phospho-ataxia telangiectasia mutated (ATM), ATM, phospho-p53, p53, phospho-PI3K, PI3K, phospho-Akt, and Akt antibodies were obtained from New England BioLabs (Hertfordshire, UK). Manganese superoxide dismutase (MnSOD), Bcl-2, BAX, cleaved caspase-3 (c-caspase-3), and poly (ADP ribose) polymerase-1 (PARP-1) were purchased from Santa Cruz Biotechnology (Delaware, CA, USA). Goat anti-rabbit or mouse IgG antibody was purchased from Jackson ImmunoResearch (West Grove, PA, USA). Lycopene and Akt inhibitor were purchased from Sigma (St. Louis, MO, USA).
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