Lp100r freeze dryer

OCE water extract (OCE–1) was prepared at KIOM according to a previously reported manufacturing method [27 (link),28 (link)]. That is, after mixing the same amount (each 625.0 g) of the eight herbal medicines shown in Table S1, 50 L of DW was added, and the mixture was extracted for 2 h at 100 °C using an electric extractor, COSMOS-660 (Kyungseo E&P, Incheon, Korea). According to the previously reported manufacturing method. Subsequently, the extracted water solution was filtered using a sieve (53 μm mesh). As a final step for producing a powder sample, the filtered extract was freeze-dried using an LP100R freeze dryer (IlShinBioBase, Yangju, Korea) (1232.6 g, yield 24.7%). Apart from the sample prepared by KIOM, the other four commercial samples (from OCE–2 to OCE–5) were purchased from different pharmaceutical companies, Kyungbang (Incheon, Korea), Jungwoo Medicines (Asan, Korea), Hankookshinyak (Nonsan, Korea), and Tsumura & Co. (Tokyo, Japan), respectively.

The Gami-soyosan decoction was prepared in the Korea Institute of Oriental Medicine (KIOM), as described in our previous study [20 (link)]. Briefly, the 12 raw herbs of Gami-soyosan (Table 1) were obtained from Kwangmyungdang Medicinal Herbs (Ulsan, Korea) and identified by Professor Jung-Hoon Kim of the School of Korean Medicine, Pusan National University (Yangsan, Korea). A voucher specimen of each raw material (from 2012–KE45–1 to KE45–12) and the Gami-soyosan decoction (2012–KE45) were stored at the KIOM. To obtain the Gami-soyosan water decoction, the mixture of 12 herbs was extracted in distilled water at 100 °C for 2 h under pressure (98 kPa) using an electric extractor (COSMOS-660; Kyungseo Machine Co., Incheon, Korea). The extract solution was freeze-dried by using a LP100R freeze dryer (IlShinBioBase, Yangju, Korea) to afford 970.4 g of the Gami-soyosan extract.