The Caspase-Glo 3/7 assay (product no. G8090, Promega, Madison, WI, USA) results in cell lysis, followed by caspase cleavage of the substrate. This liberates free aminoluciferin, which is consumed by luciferase to generate a “glowing” luminescent signal that is proportional to caspase-3 and -7 activity. The Caspase-Glo 3/7 assay stock solution was aseptically added to wells in amounts equal to 100% of the incubation volume. The luminescence after 1.5 h of incubation was measured at 495 nm wavelength using a luminometre SpectraMax L 147 and SoftMax Pro software (software version 7, Molecular Devices, San Jose, CA, USA).
Spectramax l 147
Manufactured by Molecular Devices
Sourced in United States
The SpectraMax L 147 is a luminescence microplate reader. It measures luminescence-based assays in 96- and 384-well microplates. The instrument uses a monochromator-based excitation and emission system to detect luminescent signals.
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Lab products found in correlation
3 protocols using spectramax l 147
1
Quantifying Caspase-3/7 Activity with Luminescence
2
Caspase-3/7 Activity Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The Caspase-Glo 3/7 assay (cat. no. G8090, Promega, Madison, WI, USA) was used to check caspase 3 and 7 activity. The addition of Caspase-Glo 3/7 reagent resulted in cell lysis, followed by caspase cleavage of the substrate. The substrate released aminoluciferin, which was consumed by luciferase and then generated a “glow-type” luminescent signal that is proportional to caspase 3 and 7 activity. The Caspase-Glo 3/7 assay solution was aseptically added to wells in amounts equal to 100% [v/v] of the incubation volume. After 1.5 h of incubation, absorbance was measured at 495 nm wavelength using a luminometer SpectraMax L 147 with the SoftMax Pro software (v. 7, Molecular Devices, San Jose, CA, USA).
3
Caspase-3/7 Activity Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Caspase-Glo 3/7 assay (product no. G8090, Promega, Madison, WI, USA) was used to check caspases 3 and 7 activity. Briefly, the addition of Caspase-Glo 3/7 assay resulted in cell lysis, followed by caspase cleavage of the substrate. Substrate released aminoluciferin, which was consumed by luciferase and then a “glowing” luminescent signal proportional to caspases 3 and 7 activity was generated. The Caspase-Glo 3/7 assay stock solution was aseptically added to wells in amounts equal to 100% [v/v] of the incubation volume as previously described [24 (link)]. After 1.5 h of incubation, luminescence was measured at a 495 nm wavelength using a luminometer (SpectraMax L 147) and SoftMax Pro software (software version 7, Molecular Devices, San Jose, CA, USA).
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