Data from laboratory tests, such as the levels of C-reactive protein (CRP), erythrocyte sediment rate (ESR), Calprotectin, rheumatoid factor (RF), and anti-cyclic citrullinated peptide antibody (anti-CCP), were measured on the day of clinical examination. CRP was measured using turbidimetry (Boyuan Medical Technology Co., Ltd, Shanghai, China). ESR was measured on a PRECIL-XC-40B instrument (Mindray, China). Calprotectin was measured by a commercially available calprotectin enzyme-linked immunosorbent assay (ELISA) kit according to the manufacturer's protocol (Sun Biomedical Technology Co., Ltd, Beijing, China). RF was tested by nephelometric method (Beckman Coulter, Inc., USA). Positive RF was defined as RF>20.0IU/ml. Anti-CCP antibodies were analyzed using chemiluminescence assay (Abbott GmbH & Co.KG, Wiesbaden, Germany). Positive anti-CCP was defined as anti-CCP>5.0U/ml.
Chemiluminescence assay
Manufactured by Abbott
Sourced in Germany
Chemiluminescence assay is a laboratory technique used to detect and quantify target analytes, such as proteins, nucleic acids, or small molecules, in a sample. The assay relies on the emission of light during a chemically induced reaction, which is measured and correlated to the concentration of the target analyte. This method offers high sensitivity and wide dynamic range for various applications in clinical diagnostics, drug discovery, and biological research.
Automatically generated - may contain errors
4 protocols using chemiluminescence assay
1
Biomarker Evaluation in Rheumatic Diseases
2
Hepatitis B Serological Markers
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Serum levels of HBsAg, anti-HBs, HBeAg, anti-HBe, and anti-HBc were tested by a chemiluminescence assay (Abbott Laboratories, Delkenheim, Germany).
3
Comprehensive SARS-CoV-2 Diagnostic Criteria
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SARS-CoV-2 cases were patients with clinical or epidemiological criteria and a viral antigen detection test, or presence of SARS-CoV-2 antibodies, or patients with a positive viral real-time RT-PCR test assay regardless of clinical or epidemiological criteria (according with the WHO definitions). The clinical criteria were acute onset of fever and cough (influenza-like illness) or acute onset of three or more of any signs or symptoms (fever, cough, weakness/ fatigue, headache, myalgia, sore throat, coryza, dyspnea, nausea, diarrhea or anorexia). The epidemiological criteria were contact of a probable, confirmed or linked case to a COVID-19 cluster. SARS-CoV-2 infections were diagnosed with nasopharyngeal swabs using the CDC 2019-nCoV real-time RT-PCR diagnostic panel protocol (CDC, Atlanta, Georgia, USA), Viasure® SARS-CoV-2 real-time PCR detection kit (Certest Biotec S.L., Zaragoza, Spain), Allplex™ 2019-nCoV assay (Seegene Inc, Seoul, South Korea), or AccuPower® SARS-CoV-2 multiplex real-time RT-PCR Kit (Bioneer Corporation, Daedeok-gu, South Korea. Measurement of IgG and IgM antibodies against SARS CoV-2 was through a chemiluminescence assay (Abbott Architect Assays, Chicago, Illinois). All diagnosis tests were performed in the hospital, and cases were obtained from the clinical records and laboratory databases.
4
Serum Hormone Levels in IVF Patients
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Patient serum samples were collected at three time points during IVF: on day 2 or 3 of the menstrual cycle, i.e. before gonadotropin administration; on the day of hCG/GnRHa administration, and 35–37 h after hCG/GnRHa administration. In contrast to the other sex hormones, the serum level of AMH (AMH Gen II assay, Beckman Coulter, Brea, CA) was measured by ELISA before the IVF cycle. All serum samples were collected into tubes and centrifuged at 1300 × g at 4°C for 10 min and stored at −80°C for further analyses. Serum estradiol (E2), luteinizing hormone (LH), progesterone (P4), and/or FSH levels were measured by chemiluminescence assay (Abbott Biologicals B.V., The Netherlands).
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