Immunoblotting was performed as described previously.17 (link) Isolated liver and skeletal muscle tissues were homogenized in lysis buffer. Cell lysates were heated at 100 °C for 5 min and subjected to electrophoresis on 8–15% (vol/vol) sodium dodecyl sulfate-polyacrylamide gels and transferred onto nitrocellulose membranes. Primary antibodies used were anti-LC3 (1:1000 dilution; Cell Signaling Technology Cat# 12741, RRID:AB_2617131 ), anti-p62 (1:1000 dilution; Cell Signaling Technology Cat# 5114, RRID:AB_10624872 ), anti-phospho-AMPKα (1; 1000 dilution; Cell Signaling Technology Cat# 2531, RRID:AB_330330 ), anti-AMPKα (1:1000 dilution; Cell Signaling Technology Cat# 2532, RRID:AB_330331 ), anti-phospho-p70 S6 kinase (1:1000 dilution; Cell Signaling Technology Cat# 9205, RRID:AB_330944 ), anti-p70 S6 kinase (1:1000 dilution; Cell Signaling Technology Cat# 9202, RRID:AB_331676 ), anti-GLUT4 (1:1000 dilution; Cell Signaling Technology Cat# 2213, RRID:AB_823508 ), anti-PEPCK (1:900 dilution; Abcam Cat# ab70358, RRID:AB_1925305 ), anti-G6Pase (1:500 dilution; Abcam Cat# ab133964) and β-actin (1:2000 dilution; Cell Signaling Technology Cat# 4967, RRID:AB_330288 ). The fluorescent bands were visualized using a detection system and quantified by densitometry (Amersham ECL Prime; GE Healthcare).
Anti pepck
Manufactured by Abcam
Sourced in United States
Anti-PEPCK is an antibody that recognizes the phosphoenolpyruvate carboxykinase (PEPCK) protein. PEPCK is an enzyme involved in gluconeogenesis and plays a crucial role in glucose homeostasis. This antibody can be used for the detection and quantification of PEPCK in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti p62, by Cell Signaling Technology (1 mentions)
Anti phospho ampkα, by Cell Signaling Technology (1 mentions)
Anti p70 s6 kinase, by Cell Signaling Technology (1 mentions)
Amersham ecl prime, by GE Healthcare (1 mentions)
Anti lc3, by Cell Signaling Technology (1 mentions)
Anti glut4, by Cell Signaling Technology (1 mentions)
Anti ampkα, by Cell Signaling Technology (1 mentions)
Anti phospho p70 s6 kinase, by Cell Signaling Technology (1 mentions)
Anti g6pase, by Abcam (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Avicularin, by Merck Group (1 mentions)
Lead acetate, by Merck Group (1 mentions)
Anti p erk, by Abcam (1 mentions)
Anti p eif2α, by Abcam (1 mentions)
Anti p ire1, by Abcam (1 mentions)
Anti g6pc, by Abcam (1 mentions)
Anti il 1β, by Abcam (1 mentions)
Anti tnf α, by Abcam (1 mentions)
Anti β actin, by Abcam (1 mentions)
Anti grp78, by Abcam (1 mentions)
2 protocols using anti pepck
1
Immunoblotting Analysis of Liver and Muscle Proteins
2
ER Stress Pathway Modulators in Diabetes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Avicularin (purity > 99%) and lead acetate were purchased from Sigma Chemical Co. (St. Louis, MO, USA). The anti-p-IRE1, anti-PERK, anti-p-eIF2α, anti-GRP78, anti-PYG, anti-GK, anti-PEPCK, anti-G6PC, anti-TNF-α, anti-IL-1β and anti-β-actin antibodies were provided by Abcam (Cambridge, MA, USA and Santa Cruz Biotechnology, CA, USA) [5 (link)].
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