All chemicals were used as supplied by vendors without further purification. Imidazole, Invitrogen Novex WedgeWell 14% Tris-Glycine Mini Protein Gels, Isopropy-ß-D-thiogalactopyranoside (IPTG), lysozyme, NativePAGE 4 to 16% Bis-Tris Mini Protein Gels, NativeMark Unstained Protein Standard, Spectra Multicolor Broad Range Protein Ladder, Thermo Scientific Pierce 660 nm Protein Assay Reagent, Tris base, Tris HCl, all restriction enzymes, and all cell culture media and reagents were purchased from Fisher Scientific, Inc. (USA). Gibson Assembly Master Mix was purchased from NEB (USA). Amicon Ultra 0.5 mL centrifugal units and Benzonase nuclease were purchased from MilliporeSigma (USA). BL21 (DE3) Electrocompetent Cells used for Escherichia coli expression were also purchased from MilliporeSigma (USA). Bis-tris propane from Research Products International (USA) was used for the assembly buffer. Ni-NTA agarose from Gold Biotechnology, Inc. (USA) was used for His-tagged protein purification.
Nativepage 4 to 16 bis tris mini protein gels
Manufactured by Thermo Fisher Scientific
Sourced in United States
The NativePAGE 4 to 16% Bis-Tris Mini Protein Gels are laboratory equipment used for the separation and analysis of native (non-denatured) protein samples. The gels are designed to maintain the native structure and function of proteins during electrophoresis, allowing for the analysis of protein complexes and their interactions.
Automatically generated - may contain errors
Lab products found in correlation
Nativemark unstained protein standard, by Thermo Fisher Scientific (2 mentions)
Tris base, by Thermo Fisher Scientific (1 mentions)
Lysozyme, by Thermo Fisher Scientific (1 mentions)
Gibson assembly master mix, by New England Biolabs (1 mentions)
Spectra multicolor broad range protein ladder, by Thermo Fisher Scientific (1 mentions)
Ni nta agarose, by GoldBio (1 mentions)
Pierce 660 nm protein assay reagent, by Thermo Fisher Scientific (1 mentions)
Novex wedgewell 14 tris glycine mini protein gels, by Thermo Fisher Scientific (1 mentions)
Benzonase nuclease, by Merck Group (1 mentions)
Amicon ultra 0.5 ml centrifugal units, by Merck Group (1 mentions)
Sample loading buffer, by Bio-Rad (1 mentions)
2 protocols using nativepage 4 to 16 bis tris mini protein gels
1
Optimized His-tagged Protein Purification
2
SDS-PAGE and Native PAGE Analysis
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For SDS-PAGE, 4 μg of protein samples was loaded on to a 10% TGX™ FastCast™ (Bio-Rad Laboratories, Hercules, CA, USA) polyacrylamide gel. Protein samples were prepared with 4X sample loading buffer (Bio-Rad Laboratories) for reducing conditions and with Pierce™ LDS sample buffer, non-reducing (4X) (Thermo Fisher Scientific) for non-reducing conditions. For native PAGE, protein samples were loaded on to a NativePAGE™ 4 to 16% Bis-Tris Mini Protein Gels (Thermo Fisher Scientific) with NativeMark™ Unstained Protein Standard (Thermo Fisher Scientific) and separated at 125 V for 2 h. A recombinant prolyl-4-hydroxylase (homodimer, 49.2-kDa) was used as the protein standard [22 (link)].
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