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Anti cxcr5 l138d7

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The Anti-CXCR5 (L138D7) is a monoclonal antibody that binds to the CXCR5 receptor. CXCR5 is a chemokine receptor that plays a role in the migration and positioning of B cells.

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Lab products found in correlation

Anti cd11b m1 70, by BioLegend (3 mentions) Anti cd4 gk1, by BioLegend (3 mentions) Anti b220 ra3 6b2, by BioLegend (3 mentions) Anti ifn γ xmg1, by BioLegend (2 mentions) Anti cd25 3c7, by BioLegend (2 mentions) Anti cd8a 53 6, by BioLegend (2 mentions) Anti tbet 4b10, by BioLegend (2 mentions) Anti gr 1 rb6 8c5, by BioLegend (2 mentions) Anti foxp3 mf14, by Thermo Fisher Scientific (2 mentions) Anti cd71 r17 217, by BioXCell (2 mentions) Depletion c2f2, by BD (2 mentions) Anti cd45 i3 2, by BioLegend (2 mentions) Anti cd90.1 16 10a1, by BioLegend (2 mentions) Anti gzmb gl1, by BioLegend (2 mentions) Anti mouse human cd44 im7, by BioLegend (2 mentions) Anti human mouse bcl 6 7d1, by BioLegend (2 mentions) Anti mouse ki 67 16a8, by BioLegend (2 mentions) Anti mouse tnf α mp6 xt22, by BD (2 mentions) Anti cd3 ucht, by BioLegend (2 mentions) Anti cd8 pra t8, by BioLegend (2 mentions)

4 protocols using anti cxcr5 l138d7

1

Multicolor Flow Cytometry of Immune Cells

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Fluorophore- or biotin-conjugated antibodies specific for mouse cell-surface antigens and cytokines were as follows: anti-B220 (RA3-6B2, BioLegend), anti-CD4 (GK1.5, BioLegend), anti-CD8a (53-6.7, BioLegend), anti-CD11b (M1/70, BioLegend), anti-Gr1 (RB6-8C5, BioLegend), anti-CD25 (3C7, BioLegend), anti-Foxp3 (MF14, eBioscience), anti-TER119 (TER-119, BioLegend), anti-CD71 (R17 217.1.3, BioXCell for depletion; C2F2, BD biosciences for FACS analysis), anti-CD45 (I3/2.3, BioLegend), anti-CD90.1 (16-10A1, BioLegend), anti-Gzmb (GL1, BioLegend), anti-IFN-γ (XMG1.2, BioLegend). anti-CXCR5 (L138D7, BioLegend), anti-mouse/human CD44 (IM7, BioLegend), anti-human/mouse Bcl-6 (7D1, BioLegend), anti-T-bet (4B10, BioLegend), anti-mouse Ki-67 (16A8, BioLegend), anti-mouse TNF-α (MP6-XT22, BD). For human studies, the following fluorophore- or biotin-conjugated antibodies specific for cell surface markers or cytokines were used: anti-CD3 (UCHT, BioLegend), anti-CD8 (PRA-T8, BioLegend), anti-CD45 (2D1, BioLegend), anti-CD71 (CY1G4, BioLegend), anti-CD235a (HI264, BioLegend). ROS production was measured by labelling with 2′,7′ –dichlorofluorescin diacetate using the ROS detection kit (S0033, Beyotime).
Zhao L., He R., Long H., Guo B., Jia Q., Qin D., Liu S.Q., Wang Z., Xiang T., Zhang J., Tan Y., Huang J., Chen J., Wang F., Xiao M., Gao J., Yang X., Zeng H., Wang X., Hu C., Alexander P.B., Symonds A.L., Yu J., Wan Y., Li Q.J., Ye L, & Zhu B. (2018). Late-stage tumors induce anemia and immunosuppressive extramedullary erythroid progenitor cells. Nature medicine, 24(10), 1536-1544.
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2

Multicolor Flow Cytometry of Immune Cells

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Fluorophore- or biotin-conjugated antibodies specific for mouse cell-surface antigens and cytokines were as follows: anti-B220 (RA3-6B2, BioLegend), anti-CD4 (GK1.5, BioLegend), anti-CD8a (53-6.7, BioLegend), anti-CD11b (M1/70, BioLegend), anti-Gr1 (RB6-8C5, BioLegend), anti-CD25 (3C7, BioLegend), anti-Foxp3 (MF14, eBioscience), anti-TER119 (TER-119, BioLegend), anti-CD71 (R17 217.1.3, BioXCell for depletion; C2F2, BD biosciences for FACS analysis), anti-CD45 (I3/2.3, BioLegend), anti-CD90.1 (16-10A1, BioLegend), anti-Gzmb (GL1, BioLegend), anti-IFN-γ (XMG1.2, BioLegend). anti-CXCR5 (L138D7, BioLegend), anti-mouse/human CD44 (IM7, BioLegend), anti-human/mouse Bcl-6 (7D1, BioLegend), anti-T-bet (4B10, BioLegend), anti-mouse Ki-67 (16A8, BioLegend), anti-mouse TNF-α (MP6-XT22, BD). For human studies, the following fluorophore- or biotin-conjugated antibodies specific for cell surface markers or cytokines were used: anti-CD3 (UCHT, BioLegend), anti-CD8 (PRA-T8, BioLegend), anti-CD45 (2D1, BioLegend), anti-CD71 (CY1G4, BioLegend), anti-CD235a (HI264, BioLegend). ROS production was measured by labelling with 2′,7′ –dichlorofluorescin diacetate using the ROS detection kit (S0033, Beyotime).
Zhao L., He R., Long H., Guo B., Jia Q., Qin D., Liu S.Q., Wang Z., Xiang T., Zhang J., Tan Y., Huang J., Chen J., Wang F., Xiao M., Gao J., Yang X., Zeng H., Wang X., Hu C., Alexander P.B., Symonds A.L., Yu J., Wan Y., Li Q.J., Ye L, & Zhu B. (2018). Late-stage tumors induce anemia and immunosuppressive extramedullary erythroid progenitor cells. Nature medicine, 24(10), 1536-1544.
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3

Multiparameter Flow Cytometry for Immune Cell Analysis

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Different combinations of the following fluorescence-labeled antibodies were used for flow cytometry: For analysis of DC subsets in the lymph nodes a combination of CD103 (2E7, BioLegend), CD11c (N418, BioLegend) anti-CD16/32 (93, BioLegend) and MHC-II (M5/114.15.2, BioLegend) was used. For analysis of Tfh cells and GC B cells in spleen and lymph nodes a combination of anti-CD4 (RM4-5, BioLegend), anti-CD19 (1D3, BioLegend), anti-B220 (RA3-6B2, eBioscience), anti-CD16/32 (93, BioLegend), anti-PD1 (29F.1A12, BioLegend), anti-CXCR5 (L138D7, BioLegend), anti-CD44 (IM7, BioLegend), anti-FAS (SA367H8, BioLegend), anti-IgG1 (A85-1, BD Biosciences) and anti-GL7 (GL7, BioLegend) was used.
Ye L., Schnepf D., Ohnemus A., Ong L.C., Gad H.H., Hartmann R., Lycke N, & Staeheli P. (2021). Interferon-λ Improves the Efficacy of Intranasally or Rectally Administered Influenza Subunit Vaccines by a Thymic Stromal Lymphopoietin-Dependent Mechanism. Frontiers in Immunology, 12, 749325.
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4

Multiparametric Flow Cytometry Analysis

Cited 1 time
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Single cell suspensions obtained from the intestinal mucosa or MLNs were stained and analyzed on FACSCanto II (Becton Dickinson, Franklin Lakes, NJ, USA) using FlowJo software v10.8.2 (TreeStar, Ashland, OR, USA). The following antibodies were used: anti-B220 (RA3-6B2; BioLegend), anti-CD38 (90; BioLegend), anti-CD45 (30-F11; BioLegend), anti-GL7 (GL7; BioLegend), anti-CD138 (281-2; BioLegend), anti-CD4 (GK1.5; BioLegend), anti-CXCR5 (L138D7, BioLegend), anti-PD-1 (29F.1A12, BioLegend), anti-Siglec-F (E50-2440, BD Biosciences, Franklin Lakes, NJ, USA), anti-CD11c (N418, BioLegend), anti-I-A/I-E (M5/114.15.2, BioLegend), anti-IgE (RME-1, BioLegend), anti-CD103 (W19396D, BioLegend), anti-CD11b (M1/70, BioLegend), anti-IL-7Rα (A7R34, BioLegend), anti-CCR6 (29-2L17, BioLegend), anti-TCRβ (H57-597, BioLegend), and anti-TCRγδ (UC7-13D5, BioLegend). Dead cells were gated out using a Zombie NIR Fixable Viability Kit (BioLegend). Before staining, Fc receptors were blocked with an anti-CD16/32 antibody (2.4G2, BioLegend). Negative controls consisted of isotype-matched, directly conjugated, nonspecific antibodies (BD Biosciences). Intracellular staining was performed using anti-Foxp3 antibody (FJK-16s; Thermo Fisher Scientific) and anti-RORγt antibody (Q31-378; BD Biosciences), as described previously [29 (link)].
Kurihara S., Suzuki K., Yokota M., Ito T., Hayashi Y., Kikuchi R., Kageyama T., Meguro K., Tanaka S., Iwata A., Goto Y., Suto A, & Nakajima H. (2024). Eosinophils Contribute to Oral Tolerance via Induction of RORγt-Positive Antigen-Presenting Cells and RORγt-Positive Regulatory T Cells. Biomolecules, 14(1), 89.
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