Cd45 pe 30 f11

Manufactured by Thermo Fisher Scientific

CD45-PE (30-F11) is a fluorescent-labeled antibody that binds to the CD45 protein, which is expressed on the surface of various leukocyte cell types. This product is commonly used in flow cytometry applications for the identification and analysis of different immune cell populations.

Automatically generated - may contain errors

Lab products found in correlation

Cytofix cytoperm, by BD (1 mentions) F4 80 fitc bm8, by Thermo Fisher Scientific (1 mentions) Gr1 apc cy7 rb6 8c5, by BioLegend (1 mentions) Ly6g bv510, by BioLegend (1 mentions) Lsrfortessa, by BD (1 mentions) Cd3 apc 145 2c11, by BD (1 mentions) Cd11b fitc m1 70, by BioLegend (1 mentions) Live dead yellow, by Thermo Fisher Scientific (1 mentions) Matrigel, by Corning (1 mentions) Tryple express, by Thermo Fisher Scientific (1 mentions) 7 aad, by Thermo Fisher Scientific (1 mentions) E cadherin, by Proteintech (1 mentions) N cadherin, by Proteintech (1 mentions) Vimentin, by Affinity Biosciences (1 mentions) Collagen type 1, by Proteintech (1 mentions) F actin, by Beyotime (1 mentions) Snail, by Affinity Biosciences (1 mentions) Pd l1, by Proteintech (1 mentions) Gapdh, by Proteintech (1 mentions) Anti streptavidin pe cy7, by BD (1 mentions)

Spelling variants of this product

CD45-PE (59 citations) CD45 (30-F11, (34 citations) Anti-CD45 PE (30-F11, (3 citations) Anti-CD45-PE (clone 30-F11 (2 citations) CD45-PE (clone 30-F11) (2 citations)

5 protocols using cd45 pe 30 f11

Isolation and Characterization of Murine Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Following sacrifice, the TDLN was mechanically dissociated between two frosted slides and filtered using 40-μm filters. Cells were resuspended in PAB (1L PBS, 1g sodium azide, 10 g BSA) at approximately 1 × 10⁶ cells per sample. The following antibodies were used for identification of cell subsets: CD45-PE (30-F11, eBioscience), CD11b-BV450 (M1/70, eBioscience), F4/80-FITC (BM8, eBioscience), IA/IE-PerCP-Cy5.5 (M5/114.15.2, BD), Gr1-APC-Cy7 (RB6-8C5, BioLegend). Surface markers were stained for 30 minutes at 4°C in the dark. Cells were fixed for 20 minutes using Cytofix/Cytoperm (BD), washed and resuspended in PAB. All samples were run on an Amnis ImageStream GenX (Luminex Corporation). 2–5 × 10⁴ events/sample were collected and analyzed using FlowJo software (FlowJo).

Han B.J., Murphy J.D., Qin S., Ye J., Uccello T.P., Garrett-Larsen J., Belt B.A., Prieto P.A., Egilmez N.K., Lord E.M., Linehan D.C., Mills B.N, & Gerber S.A. (2020). Microspheres Encapsulating Immunotherapy Agents Target the Tumor-Draining Lymph Node in Pancreatic Ductal Adenocarcinoma. Immunological investigations, 49(7), 808-823.

+ Open protocol

+ Expand

Multiparametric flow cytometry of immune cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single‐cell suspensions for flow cytometry were prepared as previously described (Shen et al., 2018) and detailed in Data S1. Leucocytes were stained with CD45‐PE (30‐F11; eBioscience), CD11b‐FITC (M1/70; BioLegend), CD3‐APC (145‐2C11; BD Biosciences), Ly6C‐APC Cy7 (AL‐21; BD Biosciences) and Ly6G‐BV510 (1A8; BioLegend), together with Fc receptor blocking using anti‐CD16/32 before resuspension in FACS buffer containing 2% FBS and viability dye (7‐aminoactinomycin D; 7‐AAD). The LSR‐Fortessa (BD Biosciences) and FlowJo (Tree Star) were used for acquisition/analysis.

Wen S.W., Shim R., Ho L., Wanrooy B.J., Srikhanta Y.N., Prame Kumar K., Nicholls A.J., Shen S., Sepehrizadeh T., de Veer M., Srikanth V.K., Ma H., Phan T.G., Lyras D, & Wong C.H. (2019). Advanced age promotes colonic dysfunction and gut‐derived lung infection after stroke. Aging Cell, 18(5), e12980.

+ Open protocol

+ Expand

Isolation and Culture of Intestinal Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

For intestinal stem cell (ISC) isolation, crypt suspensions were dissociated into individual cells with TrypLE Express (Invitrogen). Cells were stained with an antibody cocktail consisting of CD45-PE (30-F11, eBioscience,), CD31-PE (Mec13.3, Biolegend), CD24-Pacific Blue (M1/69, Biolegend), EpCAM-APC (G8.8, eBioscience) and Kit (Biolegend). ISCs were isolated as Lgr5-eGFP^hiEpCAM⁺CD31⁻CD45⁻ Live/Dead-yellow⁻. Paneth cells from the small intestine were isolated as CD24^hi SSC^hi c-Kit^hi Lgr5-eGFP⁻EpCAM⁺CD31⁻CD45⁻ Live/Dead-yellow⁻. Cells were sorted with a BD FACS Aria Fusion cell sorter into complete crypt medium for culture. Dead cells were excluded from the analysis with the viability dye Live/Dead yellow (Life Technologies). ISCs and Paneth cells were mixed after sorting in a 1:1 ratio, centrifuged and then seeded onto Matrigel (Corning 356231 growth factor reduced) in a flat-bottom 24-well plate. The crypt medium was changed every third day. Organoids were quantified on days 3, 7 and 10 of culture unless otherwise specified.

Dmitrieva-Posocco O., Wong A.C., Lundgren P., Golos A.M., Descamps H.C., Dohnalová L., Cramer Z., Tian Y., Yueh B., Eskiocak O., Egervari G., Lan Y., Liu J., Fan J., Kim J., Madhu B., Schneider K.M., Khoziainova S., Andreeva N., Wang Q., Li N., Furth E.E., Bailis W., Kelsen J.R., Hamilton K.E., Kaestner K.H., Berger S.L., Epstein J.A., Jain R., Li M., Beyaz S., Lengner C.J., Katona B.W., Grivennikov S.I., Thaiss C.A, & Levy M. (2022). β-Hydroxybutyrate suppresses colorectal cancer. Nature, 605(7908), 160-165.

+ Open protocol

+ Expand

Comprehensive Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The antibodies of Smad2/3 (3102, CST), AKT (#9272, CST), p-AKT (#9271, CST), p-Smad2/3 (#ab272332, Abcam), E-cadherin (#20874-1-AP, Proteintech), N-cadherin (#22018-1-AP, Proteintech), Vimentin (#BF8006, Affinity Biosciences), Collagen Type I (#14695-1-AP, Proteintech), NF-κB1 (#14220-1-AP, Proteintech), LEF-1 (#28540-1-AP, Proteintech), PD-L1 (#66248-1-Ig, Proteintech), GAPDH (#60004-1-Ig, Proteintech), SNAIL (#AF6032, Affinity Biosciences), ROCK1 (#PTM-6454, PTM Bio), ROCK2 (#PTM-6169, PTM Bio), Ki67 (#GB111499, Servicebio), and fibrillar actin (F-actin) (#C1033, Beyotime) were used for western blot, immunofluorescence staining, and immunohistochemical staining. The following antihuman antibodies were used for flow cytometry: CD45 (PE, 30-F11; eBioscience), CD8α (APC, 53-6.7; eBioscience), 7-AAD (Invitrogen), CD3 (eFluor™ 506, UCHT1; eBioscience), PD-1 (Pacific Blue, EH12.2H7; Biolegend), and TIM-3 (FITC, F38-2E2; Biolegend).

Wang P., Zhuang W., Zheng Z., Zhang L., Zhang X, & Chen Q. (2023). Dissecting T-cell heterogeneity in esophageal squamous cell carcinoma reveals the potential role of LAIR2 in antitumor immunity. Clinical and experimental immunology, 214(1).

+ Open protocol

+ Expand

Immunophenotypic Analysis of HSC Populations

Check if the same lab product or an alternative is used in the 5 most similar protocols

For analysis of AGM-HSC populations, cells were stained with either (i) cKit APC (2B8, DB Pharmingen) and CD34-PE (RAM34, BD Pharmingen), (ii) VE-Cadherin (clone 11D4.1, BD Pharmingen) followed by anti-rat Tricolour (PeCy5) antibody and CD45-PE (30-F11, eBioscience) (Invitrogen) or (iii) CD45-PE, VE-Cad:PeCy5, cKit-APC, CD34 staining the rat antimouse CD34 biotin (BD Pharmingen) followed by incubation with the secondary antibody anti-streptavidin PeCy7 (BD Pharmingen). Fetal liver cells were stained for lineage markers using purified rat antimouse antibodies the Lin-(Ter119, Gr1, CD4, CD8, B220) followed by anti-rat Tricolour (Pe-Cy5) secondary antibody. For cKit and Sca-1 staining, cKit-APC and anti-Sca-1-PE were used. . Lastly CD34 biotinylated antibody (BD Pharmingen) combined with the streptavidin PeCy7 was used for CD34 staining. For immunophenotyping of bone marrow, cells were stained for lineage analysis with purified anti mouse Ter119 (erythroid), Gr1 and Mac-1 (granulo-monocytic), B220 (B cells), CD3, CD8 and CD4 (T cell) followed by anti-rat Tricolour (PeCy5) antibody and ckit APC, Sca-1 PE and CD34 FITC. + Kit + Sca. For LT/ST HSC compartment analysis, cells were stained as before for KLS, and CD34. Cells were analysed for HSC and progenitor compartments by flow cytometry using the Cyan ADP flow cytometry platform (Beckman Coulter, High Wycombe, UK).

Migueles R.P., Shaw L., Rodrigues N.P., May G., Henseleit K., Anderson K.G., Goker H., Jones C.M., de Bruijn M.F., Brickman J.M, & Enver T. (2017). Transcriptional regulation of Hhex in hematopoiesis and hematopoietic stem cell ontogeny. Developmental biology, 424(2).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!