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2 protocols using amobarbital

1

Quantitative Analysis of Barbiturates

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Barbital, phenobarbital, amobarbital, pentobarbital, secobarbital, butalbital, thiopental sodium standard and the internal standard (IS) phenobarbital-d5 were purchased from Cerilliant Corporation (Round Rock, TX, USA). HPLC grade of acetonitrile, acetone, methanol and ammonium acetate were the productions of Fisher Scientific (Fair Lawn, NJ, USA). Ultrapure water was produced in the laboratory using a Milli-Q system (Millipore, Billerica, MA, USA).
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2

Optimized LC-MS Workflow for DOA

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Optima LC/MS grade methanol (MeOH), LC/MS grade 0.1% formic acid in water, LC/MS grade formic acid ampules, optima grade dichloromethane (DCM), optima LC/MS grade isopropanol (IPA), hydrochloric acid HCl, tert-butyl methyl ether, (MTBE), and hexane were purchased from Fisher Scientific. Ammonium hydroxide (NH4OH was purchased from Sigma. DOA analytical standards: (+/-) amphetamine, (+/-) methamphetamine, cocaine, benzoylecgonine, morphine, phencyclidine, (-) delta9-THC, (+/-) amphetamine -D5, (+/-) methamphetamine -D5, cocaine -D3, benzoylecgonine -D3, morphine -D3, phencyclidine -D5, and (-) delta9-THC -D3 were obtained as 1mg/mL solutions from Cerilliant (Round Rock, TX, USA). Interfering substances: Diphenhydramine HCl, Alprazolam, Dihydrocodeine, S-Nicotine, Caffeine, Cortisol, -Cotinine, Dextromethorphan, Ibuprofen, Naproxen, Prednisone, R-Pseudoephedrine, Amobarbital, Propranolol, Nicotinamide, Carbamazepine, Clobazam, Clonazepam, Valproic Acid, Verapamil, and Sertraline were purchased from Cerilliant (Round Rock, TX, USA). Stabilization buffer was purchased from Immunalysis (Pomona, CA). The Raptor Bipheyl LC column; 2.7um, 100mm x 2.1mm was purchased from Restek (Bellefonte, PA). Isolute SLE+ columns were purchased from Biotage (Charlotte, NC). The PRESSURE+ 48 positive pressure manifold was obtained from Biotage (Charlotte, NC).
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