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Xanthine oxidase activity assay kit

Manufactured by Abcam
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The Xanthine Oxidase Activity Assay Kit measures the activity of the enzyme xanthine oxidase in biological samples. The kit provides the necessary reagents and protocols to quantify xanthine oxidase levels using a colorimetric method.

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Lab products found in correlation

Lipid peroxidation microplate assay kit, by Oxford Biomedical Research (1 mentions) Potassium oxonate, by Merck Group (1 mentions) Allopurinol, by Merck Group (1 mentions) Protease and phosphatase inhibitor, by Thermo Fisher Scientific (1 mentions) Amersham ecl system, by GE Healthcare (1 mentions) β actin antibody, by Santa Cruz Biotechnology (1 mentions) Primescript rt reagent kit with gdna eraser and tb green premix ex taq 2 tli rnaseh plus, by Takara Bio (1 mentions) Anti glut9, by Abcam (1 mentions) Rna isolation kit, by Promega (1 mentions) Beadbug microtube homogenizer, by Benchmark Scientific (1 mentions) 3 mm zirconium beads, by Benchmark Scientific (1 mentions) Uric acid assay kit, by Abcam (1 mentions) Superoxide dismutase activity colorimetric assay kit, by Abcam (1 mentions)

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Activity assay kits (2 citations)

5 protocols using xanthine oxidase activity assay kit

1

Renal Lipid Peroxidation and Xanthine Oxidase

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Levels of malondialdehyde (MDA) and 4‐hydroxynonenal (4HNE), indicators of lipid peroxidation, and xanthine oxidase activity in renal tissues were measured using a Lipid Peroxidation Microplate Assay Kit (FR12; Oxford Biomedical Research, Oxford, MI, USA) and a Xanthine Oxidase Activity Assay Kit (ab102522; Abcam, Cambridge, UK), respectively.
Kimura Y., Kuno A., Tanno M., Sato T., Ohno K., Shibata S., Nakata K., Sugawara H., Abe K., Igaki Y., Yano T., Miki T, & Miura T. (2019). Canagliflozin, a sodium–glucose cotransporter 2 inhibitor, normalizes renal susceptibility to type 1 cardiorenal syndrome through reduction of renal oxidative stress in diabetic rats. Journal of Diabetes Investigation, 10(4), 933-946.
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2

Uric Acid Metabolism Regulation Protocol

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LBPs (≥90%) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). Potassium oxonate (97%) and allopurinol (≥99%) was purchased from Sigma-Aldrich Co. LLC (Shanghai, China). Uric acid (UA), serum creatinine (SCR), blood urine nitrogen (BUN) and xanthine oxidase activity assay kit were purchased from Abcam Inc. (Cambridge, UK). The RNA isolation kit was obtained from Promega Biotechnology Co., Ltd. (Beijing, China). PrimeScriptTM RT reagent kit with gDNA Eraser and TB Green® Premix Ex Taq™ II (Tli RNaseH Plus) was purchased from Takara Biomedical Technology Co., Ltd. (Dalian, China). Protease and phosphatase inhibitors were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Anti-OAT1, anti-OAT3 and β-actin antibody were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Anti-GLUT9 was purchased from Abcam Inc. (Cambridge, UK). The Amersham ECL System was purchased from GE Healthcare (Pittsburgh, PA, USA). Other biochemical reagents and chemicals were of analytical grade.
Yu X., Zhang L., Zhang P., Zhi J., Xing R, & He L. (2020). Lycium barbarum polysaccharides protect mice from hyperuricaemia through promoting kidney excretion of uric acid and inhibiting liver xanthine oxidase. Pharmaceutical Biology, 58(1), 944-949.
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3

Xanthine Oxidase Activity Assay

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Tumors were dissected away from surrounding fat 24 hours post-treatment and lysed in XO assay buffer (Abcam) using 3 mm zirconium beads (Benchmark Scientific) in the BeadBug Microtube Homogenizer (Benchmark Scientific) for 2 cycles of 45 seconds at 3000 rpm. Crude lysate was centrifuged for 10 minutes at 16000 × g at 4°C to obtain clarified lysate. XO activity of the lysate was determined using the Xanthine Oxidase Activity Assay kit (Abcam), setting up the fluorometric assay and performing calculations according to the manufacturer’s instructions, and reading fluorescence with a Victor X4 fluorescence microplate reader.
Linde I.L., Prestwood T.R., Qiu J., Pilarowski G., Linde M.H., Zhang X., Shen L., Reticker-Flynn N.E., Chiu D.K., Sheu L.Y., Van Deursen S., Tolentino L.L., Song W.C, & Engleman E.G. (2023). Neutrophil-activating therapy for the treatment of cancer. Cancer cell, 41(2), 356-372.e10.
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4

Lipid and Enzymatic Profile Assay

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Total cholesterol (TC), triglyceride (TG), and high-density lipoprotein cholesterol (HDL-c) concentrations were determined using specific commercial kits (Human Gesellschaft fuer Biochemica und Diagnostica mbH; Wiesbaden, Germany). Aspartate transaminase (AST) and alkaline phosphatase (ALP) levels were measured by the Clinical Chemistry Laboratory Unit of the Faculty of Associated Medical Sciences, Khon Kaen University, Thailand. Serum xanthine oxidase activity was determined using the Xanthine Oxidase Activity Assay Kit (ab102522, Abcam Plc; Cambridge, UK). Serum uric acid levels were determined using the Uric Acid Assay Kit (ab65344, Abcam Plc).
Potue P., Chiangsaen P., Maneesai P., Khamseekaew J., Pakdeechote P., Chankitisakul V., Boonkum W., Duanghaklang N, & Duangjinda M. (2022). Effects of Thai native chicken breast meat consumption on serum uric acid level, biochemical parameters, and antioxidant activities in rats. Scientific Reports, 12, 14056.
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5

Superoxide Dismutase Activity in Vitreous

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Human post mortem vitreous base was dissected to obtain 50 mg pieces. The samples were incubated either with 250 μL of 50 mM D-glucose (n = 10) or phosphate buffer alone (n = 5) at room temperature for 45 min. Following incubation, samples were spun at 1000 g for 5 min. The supernatant was collected, being careful to avoid the vitreous base matrix at the bottom of the tube and applied to the Superoxide Dismutase Activity Colorimetric Assay Kit (Abcam product #ab65354). Activity is indicative of SOD3 released from the vitreous base into the supernatant solution. Xanthine Oxidase was measured using the Xanthine Oxidase Activity Assay Kit (Abcam; ab102522).
Wert K.J., Velez G., Cross M.R., Wagner B.A., Teoh-Fitzgerald M.L., Buettner G.R., McAnany J.J., Olivier A., Tsang S.H., Harper M.M., Domann F.E., Bassuk A.G, & Mahajan V.B. (2018). Extracellular superoxide dismutase (SOD3) regulates oxidative stress at the vitreoretinal interface. Free radical biology & medicine, 124, 408-419.
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