Dm il led inverted

Hemocytes and HPT cells were isolated as above and attached to carefully washed and autoclaved coverslips in 24-well plates. After attachment the cells were fixed in 3.7% formalin in CFS for 10 min at room temperature. The formalin was removed and exchanged for 70% ethanol and the plates were stored at −20°C overnight. The plates were then used for fluorescent RNA in situ hybridization using the QuantiGene ViewRNA ISH Cell assay kit and procedure according to the manufacturer’s user manual. The cells were finally mounted in Prolong® Gold Antifade Reagent and analyzed using fluorescence microscopy with a Leica DM IL Led inverted (Figure 8), a Leica DM5500B upright microscope (Figures 5, 6, and 9).

Cultured or FACSorted cells were treated and stained with antibodies (Table S1) as described [32 (link)] for IF studies using Leica DM IL LED inverted fluorescent microscopy.