A hydroalcoholic extract of seeds of S. cumini (Batch no. SC/11LOT001, Mfg. Aug 2013) was obtained from Natural Remedies (Bangaluru, India) as a gift sample in July 2014. Acetonitrile (HPLC grade) and potassium dihydrogen phosphate were purchased from Merck Specialist Pvt. Ltd. (Mumbai, India). Methanol (HPLC grade), acetic acid (HPLC grade) and maltose monohydrate were purchased from S.D. Fine-chem Ltd. (Mumbai, India). Gallic acid and formic acid were purchased from Loba Chemie Pvt. Ltd. (Mumbai, India). Ellagic acid was purchased from TCI Chemicals Pvt. Ltd. (Chennai, India).
Rat intestinal acetone powder, acarbose, ferulic acid, sinapic acid and kaempferol were purchased from Sigma–Aldrich (Bangaluru, India). Cinnamic acid was purchased from Fisher Scientific (Mumbai, India). Quercetin was purchased from Otto Chemie Pvt Ltd (Mumbai, India). Glucose level was estimated by using commercially available kit (Erba diagnostics Mannheim GmbH, Germany). Triple distilled water was produced in laboratory for preparation of all the solutions.
Rat intestinal acetone powder, acarbose, ferulic acid, sinapic acid and kaempferol were purchased from Sigma–Aldrich (Bangaluru, India). Cinnamic acid was purchased from Fisher Scientific (Mumbai, India). Quercetin was purchased from Otto Chemie Pvt Ltd (Mumbai, India). Glucose level was estimated by using commercially available kit (Erba diagnostics Mannheim GmbH, Germany). Triple distilled water was produced in laboratory for preparation of all the solutions.