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Ready set go elisa sets

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Ready-Set-Go ELISA Sets are a product line of enzyme-linked immunosorbent assay (ELISA) kits designed for the detection and quantification of various analytes. These kits provide the necessary reagents and materials to perform ELISA experiments, including pre-coated plates, detection antibodies, and other essential components.

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ELISA Ready-SET-Go (110 citations) Ready-SET-Go (98 citations) ELISAs (35 citations)

4 protocols using ready set go elisa sets

1

Quantification of Human Cytokines

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The concentrations of human IL-1β and CXCL8/IL-8 proteins in the supernatants of stimulated cells were determined with Ready-Set-Go ELISA Sets (Thermo Fisher Scientific, Life Technologies SAS, Courtaboeuf, France) according to the manufacturer’s instructions. We used serial dilutions of recombinant human IL-1β and CXCL8/IL-8 to generate a standard curve. We determined optical density at 450 nm, with wavelength correction at 570 nm.
Grange P.A., Ollagnier G., Beauvais Remigereau L., Nicco C., Mayslich C., Marcelin A.G., Calvez V, & Dupin N. (2022). A New Topical Candidate in Acne Treatment: Characterization of the Meclozine Hydrochloride as an Anti-Inflammatory Compound from In Vitro to a Preliminary Clinical Study. Biomedicines, 10(5), 931.
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2

Quantifying CXCL8/IL-8 and TNF-α Proteins

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Human CXCL8/IL-8 and TNF-α protein concentrations were measured in the supernatants of stimulated cells or skin explants with Ready-Set-Go ELISA Sets (Thermo Fisher Scientific, Life Technologies SAS, Courtaboeuf, France) according to the manufacturer’s instructions. We used serial dilutions of recombinant human CXCL8/IL-8 and TNF-α for the establishment of standard curves. Optical density at 450 nm was determined with a wavelength correction of 570 nm.
Mayslich C., Grange P.A., Castela M., Marcelin A.G., Calvez V, & Dupin N. (2022). Characterization of a Cutibacterium acnes Camp Factor 1-Related Peptide as a New TLR-2 Modulator in In Vitro and Ex Vivo Models of Inflammation. International Journal of Molecular Sciences, 23(9), 5065.
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3

Quantification of Acute Phase CRP and IL-8

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The ELISA for the determination of the acute phase C-reactive protein (CRP) was performed as described in [29 (link)]. IL-8 cytokine levels in plasma and supernatants were determined using a kit from eBioscience (Ready-SET-Go!ELISA Sets), according to the manufacturers’ protocol.
Takele Y., Adem E., Getahun M., Tajebe F., Kiflie A., Hailu A., Raynes J., Mengesha B., Ayele T.A., Shkedy Z., Lemma M., Diro E., Toulza F., Modolell M., Munder M., Müller I, & Kropf P. (2016). Malnutrition in Healthy Individuals Results in Increased Mixed Cytokine Profiles, Altered Neutrophil Subsets and Function. PLoS ONE, 11(8), e0157919.
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4

Quantifying Cytokine Levels by ELISA

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To quantify the concentrations of IL-22, IFN-γ and IL-17A in sera and cell supernatants, Ready-SET-Go ELISA Sets from eBioscience were used according to the manufacturer’s instructions.
Schmalzl A., Leupold T., Kreiss L., Waldner M., Schürmann S., Neurath M.F., Becker C, & Wirtz S. (2022). Interferon regulatory factor 1 (IRF-1) promotes intestinal group 3 innate lymphoid responses during Citrobacter rodentium infection. Nature Communications, 13, 5730.
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