Rabbit anti rat nrf2 polyclonal antibody

STZ and TTC were purchased from Sigma-Aldrich Co. (Saint Louis, USA). Lyophilizing rhGLP-1 (7-36) powder, manufactured by Shanghai Huayi Bio-Technology Co., Ltd (Shanghai, China), was diluted in saline. Nimodipine injection was purchased from Byer Schering Pharma AG (Beijing, China). Insulin Aspart 30 Injection was from Novo Nordisk Company. Rabbit anti-rat P-PI3K monoclonal antibody (No: 4228P) and rabbit anti-rat PI3K monoclonal antibody (No: 4257S) were purchased from Cell Signaling. Rabbit anti-rat Nrf2 polyclonal antibody (No: 31163), GAPDH monoclonal antibody (No: 128915), and HRP-Goat Anti rabbit IgG (No: 6721) were purchased from Abcam. Rabbit anti-rat HO-1 monoclonal antibody (No: 2322-1) was purchased from EPITOMICS. The ELISA kits for detecting MDA and SOD were from American BIO-RAD Company.

RA (specification: 1 g), SFN (specification: 5 mg), and dimethyl sulfoxide (DMSO) (specification: 100 mL/bottle) were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). The following primary antibodies were used: rabbit anti-rat Nrf2 polyclonal antibody and goat anti-rat HO-1 polyclonal antibody purchased from Abcam (Cambridge, UK); and rabbit anti-rat NF-κB polyclonal antibody and rabbit anti-rat tumor necrosis factor (TNF-α) polyclonal antibody purchased from Cell Signaling Technology (Beverly, MA, USA). The following secondary antibodies were used: dog anti-rabbit polyclonal antibody labeled with Cy3 fluorescein and dog anti-goat polyclonal antibody labeled with fluorescein isothiocyanate, which were purchased from Abcam; and rabbit anti-rat β-actin polyclonal antibody, which was purchased from Abcam. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS–PAGE) gel preparation kit was purchased from Beyotime Institute of Biotechnology (Shanghai, People’s Republic of China). Instruments included the following: animal skull stereotaxic apparatus, electronic analytical balance, image analyzer, and ultraviolet spectrophotometer.