Blood was collected in EDTA collection tubes (Greiner Bio-One, Germany), and plasma was obtained by centrifugation at 2000xg for 10 minutes. ALT plasma levels were determined using Reflotron ALT strips on a Reflotron Plus (Roche). Plasma and hepatic triglyceride and cholesterol levels were measured according to the manufacturer’s instructions using Liquid Reagents kit (GPO-PAP Triglyceride Liquicolor kit, CHOD-PAP Cholesterol Liquicolor kit, HUMAN Biochemica, and Diagnostica mbH, Wiesbaden, Germany). Protein content was measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). TBARS were assessed following the manufacturer’s instructions using the Quantichrome DTBA-100 kit (BioAssay Systems, USA). Plasma ethanol levels were determined using the Ethanol assay kit (MAK076-1KT, Sigma-Aldrich, Vienna, Austria) according to the manufacturer’s protocol.
Edta collection tubes
Manufactured by Greiner
Sourced in Germany, Belgium
EDTA collection tubes are used to collect and store blood samples. EDTA (Ethylenediaminetetraacetic acid) is an anticoagulant that prevents blood from clotting. These tubes are designed to maintain the integrity of the blood sample for various laboratory analyses.
Automatically generated - may contain errors
2 protocols using edta collection tubes
1
Plasma Biomarker Quantification Protocol
2
Plasma Separation from Peripheral Blood
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The research related to human use has been complied with all the relevant national regulations, institutional policies and in accordance the tenets of the Helsinki Declaration, and has been approved by the University of Antwerp ethics committee (Belgian registration number: B300201214328). All healthy individuals gave their written informed consent.
Peripheral venous blood was drawn into pre-chilled EDTA collection tubes (Greiner Bio-One, Vilvoorde, Belgium) and quickly mixed by inversion. The plasma was separated from blood cells within 90 min by centrifugation at 4 °C, 1,200 x g for 15 min, and the supernatant was aliquoted and stored frozen at -80 °C.
Peripheral venous blood was drawn into pre-chilled EDTA collection tubes (Greiner Bio-One, Vilvoorde, Belgium) and quickly mixed by inversion. The plasma was separated from blood cells within 90 min by centrifugation at 4 °C, 1,200 x g for 15 min, and the supernatant was aliquoted and stored frozen at -80 °C.
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