DTX was supplied by Qilu Pharmaceutical (Jinan, China). DSPE-PEG2K was purchased from NOF (Tokyo, Japan).
DSPE-PEG5K was obtained from Avanti Polar Lipids. (Alabaster, AL). 3,3′-Dioctadecyloxacarbocyanine perchlorate (DIO), nystatin, and
sucrose were obtained from Sigma-Aldrich (St. Louis, MO). 1,1’-Dioctadecyltetramethyl indotricarbocyanine iodide) (DIR) was purchased from Perkin-Elmer (Waltham, MA).
Soybean lecithin (Lipoid S-100) was obtained from Lipoid GmbH (Ludwigshafen, Germany).
Chlorpromazine and
cytochalasin D were supplied by Cayman Chemicals (Ann Arbor, MI).
Dynasore was obtained from MedChem Express (Monmouth Junction, NJ).
LysoTracker Red DND-99 and
Hoechst3342 were obtained from Thermo Fisher Scientific (Waltham, MA). The anti-PEG backbone mAb AGP4 was provided by Dr. Steve R. Roffler (Academia Sinica, Taipei, Taiwan). Tynen
® (solvent-based DTX) is a generic product of DTX manufactured by TYY Pharmaceutical (lot no: STW1407, Taipei, Taiwan). All reagents were of analytical grade and solvents used in the high-performance liquid chromatography (HPLC) or ultra-performance liquid chromatography (UPLC)/mass spectrometry (MS/MS) analysis was of HPLC or MS grade.
Su C.Y., Chen M., Chen L.C., Ho Y.S., Ho H.O., Lin S.Y., Chuang K.H, & Sheu M.T. (2018). Bispecific antibodies (anti-mPEG/anti-HER2) for active tumor targeting of docetaxel (DTX)-loaded mPEGylated nanocarriers to enhance the chemotherapeutic efficacy of HER2-overexpressing tumors. Drug Delivery, 25(1), 1066-1079.
