Anti cd69

Multiplex immunofluorescence staining assays were performed to validate the existence of CD8+Trm cells in oral mucosal tissue of OLP patients and healthy controls. The tissue sections were incubated with the primary antibody anti-CD8 (rabbit; Abcam, USA; Cat. no. ab237709;1:1000) for 1h at room temperature and anti-CD69 (rabbit; Abcam, USA; Cat. no. ab233396;1:500) for overnight at 4°C. Then these sections were incubated with secondary antibody Opal Anti-Ms + Rb HRP (Akoya Biosciences,USA; Cat. No. ARH1001EA). Images were collected using Vectra 3 Pathology Imaging System (Akoya Biosciences,USA).

CBC was measured by an automated blood count (Hemavet System 950FS). May-Grünwald-Giemsa staining (Thermo Fisher Scientific, catalog 22-050-272) was used to stain PB smears. Histological analyses were performed as previously described (83 (link)), and samples were stained with H&E.
The following antibodies were used for IHC analysis: anti-CD68 (Leica Biosystems, PA0273); anti-CD69 (Abcam, 202909); anti-Ki67 (Cell Signaling Technology, 12202S; Leica Biosystems, PA0230) anti-galectin/MAC2 (Abcam, ab76245); anti-CD3 (Cell Signaling Technology, 99940S); anti-CD4 (Leica Biosystems, PA0427); anti-CD8 (Leica Biosystems, PA0183); and anti-FOXP3 (Abcam, ab215206). For BM and liver fibrosis analyses, a reticulin staining kit (MilliporeSigma, HT102A-1KT) was used.
For plasma analyses, PB samples were collected in heparinized tubes and centrifuged, and plasma was frozen or directly assayed. Plasma was used for the cytokine array analyses (R&D Systems, ARY028); the ferritin ELISA kit (ALPCO, 41-FERMS-E01); the sCD25 ELISA kit (G-Biosciences, IT5809); and chemistry profiling (HESKA; catalog 6330, COMP/EWRAP).