Clone 8e11

Manufactured by Genentech

The Clone 8E11 is a laboratory instrument designed for cell culture applications. It provides a controlled environment for the growth and maintenance of cells. The core function of the Clone 8E11 is to facilitate cell culture procedures through temperature, humidity, and gas regulation.

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Lab products found in correlation

Gp120 10e7.1d2, by Genentech (1 mentions)

Spelling variants of this product

Monoclonal anti-IL-22 (Clone 8E11) blocking antibody (2 citations) Monoclonal anti-IL-22 (clone 8E11, (2 citations)

4 protocols using clone 8e11

Naïve T Cell Transfer Colitis

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For naïve T cell transfer colitis, 4 × 10⁵ CD4⁺CD25⁻CD45RB^hi T cells were injected i.p. into Rag1^−/− or Il23a^−/−Rag1^−/− recipients. In co-transfer experiments, 2 × 10⁵ CD4⁺CD25⁻CD45RB^hi T cells from each source were mixed and injected i.p. into Rag1^−/− hosts. Where indicated, mice were injected with anti-IL-17A (1 mg per mouse every 3 days, clone 17F3 BioXCell), anti-IL-22 (0.125 mg per mouse every 3 days, Genentech, clone 8E11) or isotype control for the duration of the experiment. Mice were killed at indicated time points or killed when weight loss approached 20% of the original body weight at the start of the experiment.

Krausgruber T., Schiering C., Adelmann K., Harrison O.J., Chomka A., Pearson C., Ahern P.P., Shale M., Oukka M, & Powrie F. (2016). T-bet is a key modulator of IL-23-driven pathogenic CD4+ T cell responses in the intestine. Nature Communications, 7, 11627.

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Cytokine Neutralization for Superinfection

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Cytokine neutralization was performed by administering a total of 100 μg of anti‐IL‐17A monoclonal antibody (BioXCell, Clone 17F3), 250 μg of anti‐IL‐22 monoclonal antibody (Genentech, Clone 8E11) or equal amounts of mouse IgG1 isotype control antibody (Genentech) per mouse on each dose. For depletion of IL‐17 and IL‐22 in post‐secondary bacterial superinfection, antibody treatments were given every other day from 2 days before bacterial infection (or day 5 post‐influenza virus infection). Treatments were administered through both intranasal (20 μl) and intraperitoneal (200 μl) routes. However, after day 7 post‐influenza virus infection where lungs are severely inflamed, the full dose of antibody was administered intraperitoneally in 200 μl volume.

Er J.Z., Koean R.A, & Ding J.L. (2018). Loss of T‐bet confers survival advantage to influenza–bacterial superinfection. The EMBO Journal, 38(1), e99176.

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Indole Modulates Intestinal Permeability

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Neonatal Abx-treated mice were gavaged i.g. once daily with 100μL of H₂O or indole (10 μg/mL) from weaning until 6 days post weaning before assessment of intestinal permeability on day 7 using the FITC-dextran assay. For experiments using IL-22 neutralizing antibodies, the mice were also i.p. injected with 150μL of either PBS, isotype control (1μg/μL, GP120 10E7.1D2, Genentech) or neutralizing anti-IL-22 antibody (1μg/μL, clone 8E11, Genentech) starting day 1 post weaning and continuing on days 3 and 5. In these experiments, daily H₂O or indole gavages ended on day 5 and the FITC-dextran assay was performed on day 6.

Amano H., Yamamoto H., Senba M., Oishi K., Suzuki S., Fukushima K., Mukaida N., Matsushima K., Eguchi K, & Nagatake T. (2000). Impairment of Endotoxin-Induced Macrophage Inflammatory Protein 2 Gene Expression in Alveolar Macrophages in Streptozotocin-Induced Diabetes in Mice. Infection and Immunity, 68(5), 2925-2929.

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Cytokine Modulation in Hematopoietic Cell Transplant

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Anti-mouse IL-22 (Genentech Clone 8E11, mouse IgG1) was administered via intraperitoneal (i.p.) injection at 150 µg every 3 days from day 12 to day 21 after HCT (Fig. 2, 3) or every other day from day 0 to day 8 after HCT (Figs. 5, 6, supplemental Figs. 4 and 7). Certain control groups also received isotype control IgG1 mAb. Anti-mouse IFN-γ (Bio-x-cell, clone R4-6A2) was administered via i.p. injection at 1 mg every day from day 0 to day 5 after HCT. Anti-mouse CD4 (Bio-x-cell, clone GK1.5) was administered via i.p. injection at 500 µg on the day of HCT.

Song Q., Wang X., Wu X., Kang T.H., Qin H., Zhao D., Jenq R.R., van den Brink M.R., Riggs A.D., Martin P.J., Chen Y.Z, & Zeng D. (2021). IL-22-dependent dysbiosis and mononuclear phagocyte depletion contribute to steroid-resistant gut graft-versus-host disease in mice. Nature Communications, 12, 805.

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