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2 protocols using oroxylin a

1

Comprehensive Flavonoid Analysis Protocol

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Acetonitrile and methanol of HPLC grade were purchased from Sigma-Aldrich (St. Louis, MO, USA). Quercetin 3-methyl ether, eriodictyol, ampelopsin (dihydromyricetin), taxifolin (dihydroquercetin), aromadendrin (dihydrokaempferol), tamarixetin, quercetin 3,3'-dimethyl ether, isokaempferide, chrysoeriol, homoeriodictyol, and isoferulic acid were purchased from BioBioPha Co., Ltd (Kunming, China), and oroxylin A was purchased from Shanghai Yuanye Bio-Technology Co., Ltd (Shanghai, China). Laricitrin, syringetin, and tricetin were from Extrasynthese (Lyon, France), and tricin was from ChromaDex (Irvine, CA, USA). Quercetin, isorhamnetin, kaempferol, luteolin, baicalein, eriocitrin, SAM, and formic acid for HPLC were obtained from Aladdin (Shanghai, China). Myricetin, apigenin, and 7,8-dihydroxyflavone hydrate were purchased from TCI (Shanghai, China). Caffeic acid, naringenin, hesperetin, neoeriocitrin, and ferulic acid were also purchased from Sigma-Aldrich.
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2

Acquisition of Bioactive Compounds

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Quercetin, eriodictyol, eriocitrin, neoeriocitrin, kaempferol, luteolin, apigenin, oroxylin A, baicalein 7-O-methyl ether, gallic acid, feruloyl-CoA, and scopoletin were purchased from Shanghai Yuanye Biotechnology Co., LTD (Shanghai, China). Baicalein, isorhamnetin, and SAM were purchased from Aladdin (Shanghai, China). Chrysoeriol and homoeriodictyol were purchased from BioBioPha (Kunming, China). 7,8-dihydroxyflavone and 3′,4′-dihydroxyflavone were purchased from TCI (Shanghai, China). 8-Hydroxy-7-methoxyflavone was purchased from Biosynth (St. Gallen, Switzerland) and caffeic acid was purchased from Sigma-Aldrich (St. Louis, MO, USA). Esculetin was purchased from SinoStandards (Chengdu, China) and caffeoyl-CoA was purchased from ZZStandard (Shanghai). The chromatographic grade acetonitrile and methanol for HPLC were purchased from Sigma-Aldrich.
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