Anti nf at

Manufactured by Santa Cruz Biotechnology

Anti-NF-AT is a laboratory reagent used for the detection and study of the Nuclear Factor of Activated T-cells (NF-AT) protein. NF-AT is a family of transcription factors that play a crucial role in the regulation of immune response and T-cell activation. This product provides researchers with a specific tool to investigate the expression and function of NF-AT in various experimental systems.

Automatically generated - may contain errors

Lab products found in correlation

Anti nf κb, by Santa Cruz Biotechnology (1 mentions) Anti il 10, by Santa Cruz Biotechnology (1 mentions) Anti itpr3, by BD (1 mentions) Novolink polymer detection kit, by Leica Biosystems (1 mentions) Anti ssdna, by Abcam (1 mentions) 5 methylcytosine 5mc, by Zymo Research (1 mentions)

2 protocols using anti nf at

Immunohistochemical Analysis of NFκB, NFAT, and IL-10

Check if the same lab product or an alternative is used in the 5 most similar protocols

For immunohistochemistry analysis, all samples were previously submitted to deparaffinization. Endogenous peroxidase activity was blocked with H₂O₂ 3% three times during 10 minutes. Samples were then washed and blocked with bovine serum albumin 10% during 1 hour and then incubated with primary rabbit anti-mouse IgG antibodies: i) anti-NFκB (Santa Cruz, CA) at 1:500; ii) anti-NF-AT 1:500 (Santa Cruz, CA) and iii) anti-IL-10 (Santa Cruz, CA) at 1:500 during 2 hours at room temperature. Samples were washed twice with TBS- BSA 10% and then incubated with secondary antibody goat anti-Rabbit IgG at 1:1000 for 1 hour. After washing samples twice with BSA 2% diaminobenzidine (DAB) was added. Finally samples were washed again and counter stained with H&E. Slides were analyzed under light microscope on a blind fashion.

Peron J.P., de Brito A.A., Pelatti M., Brandão W.N., Vitoretti L.B., Greiffo F.R., da Silveira E.C., Oliveira-Junior M.C., Maluf M., Evangelista L., Halpern S., Nisenbaum M.G., Perin P., Czeresnia C.E., Câmara N.O., Aimbire F., Vieira R.D., Zatz M, & Ligeiro de Oliveira A.P. (2015). Human Tubal-Derived Mesenchymal Stromal Cells Associated with Low Level Laser Therapy Significantly Reduces Cigarette Smoke–Induced COPD in C57BL/6 mice. PLoS ONE, 10(8), e0136942.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Ischemia-Reperfusion

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunohistochemistry was performed with the Novolink Polymer Detection Kit (Leica Biosystems) using formalin-fixed, paraffin-embedded tissue slices of mice and human liver specimens from control and ischemia-reperfusion groups. Specimens were incubated with anti-ITPR3 (BD transduction Laboratories), anti-NFAT (Santa Cruz), 5 methylcytosine (5mc) (Zymo Research) and anti-ssDNA (Abcam) antibodies overnight at room temperature. The reactions were developed by applying 3,3’-diaminobenzidine (DAB). To compare ITPR3 staining in hepatic tissue, digital images were analyzed in a blinded fashion. The average pixel intensity was quantified in specimens using Image J software. The specificity of anti-ITPR3 was confirmed by observing apical labeling of cholangiocytes in all analyzed liver specimens, because this is the subcellular distribution of ITPR3 that has been established previously in these cells (19 (link)). The ITPR3 intensity value of IR groups was normalized according to control. For NFAT and ssDNA labeling analysis, cells with positive nuclear staining also were counted in a blinded fashion, being expressed as percentage of positive cells (15 (link)).

Lima Filho A.C., França A., Florentino R.M., dos Santos M.L., de Oliveira Lemos F., Missiaggia D.G., Fonseca R.C., Oliveira A.G., Ananthanarayanam M., Guerra M.T., de Castro Fonseca M., Teixeira Vidigal P.V., Lima C.X., Nathanson M.H, & Leite M.F. (2020). Inositol 1,4,5-Trisphosphate Receptor type 3 plays a protective role in hepatocytes during hepatic ischemia-reperfusion injury. Cell calcium, 91, 102264.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!