Csb e08300m

Aβ ELISA kits were used to measure Aβ42 (catalog no.: CSB-E10787m, Cusabio, China) and Aβ40 (catalog no.: CSB-E08300m, Cusabio) levels. In short, the tissues were ultrasonically lysed in PBS liquid and centrifuged at 12,000 rpm at 4 °C for 20 min. The supernatant of the lysate was transferred to a 96-well plate. It was incubated with the secondary antibody at room temperature for 1 h, and the optical density was measured at 450 nm.

The concentrations of Aβ1-42 (CSB-E10787m, Cusabio, Hubei, China), Aβ1-40 (CSB-E08300m,Cusabio), IL-1β (SEKM-0002, Solarbio, Beijing, China), and TNF-α (SEKM-0034, Solarbio) were assessed by enzyme-linked immunosorbent (ELISA) assay kits. In brief, cells were seeded onto 96-well plates and treated under different conditions. Thereafter, 100 μL of supernatants was harvested to perform ELISA assays. Nitric oxide levels were detected according to total nitrate/nitrite ratios in the supernatants following the manufacturer’s instructions (Nitric Oxide Assay Kit, EMSNO, Pierce, Rockford, IL). Briefly, nitrate reductase and the enzyme cofactor were added to wells and incubated to convert nitrate to nitrite. Next, the enhancers were added and incubated at room temperature. Finally, the Griess reagents were added and the samples were analyzed with a microplate reader.