Vector 22 fourier transform infrared ftir spectrometer

Melting points of the compounds were recorded on an XT-4 binocular microscope melting point apparatus (Beijing Tech Instruments Co., Beijing, China), and are uncorrected. Proton nuclear magnetic resonance (NMR) spectra were determined at 500 and 125 MHz using an ECX 500 NMR spectrometer (JEOL, Tokyo, Japan) in CDCl₃ solvent, using TMS as an internal standard. Infrared (IR) spectra were obtained on a Vector 22 Fourier transform infrared (FTIR) spectrometer (Bruker, Karlsruhe, Germany) in KBr disks. Elemental analyses were determined on an Elementar Vario-III CHN analyzer (Elementar Analysensysteme GmbH, Frankfurt, Germany). High resolution mass spectrometer (HRMS) data was conducted using a Thermo Scientific Q Exactive (Thermo, Waltham, MA, USA). Reaction progress was monitored by thin-layer chromatography (TLC) on silica gel GF254. Column chromatographic purification was carried out using silica gel (200–300 mesh, Qingdao Bangkai Hi-Tech materials Co., Ltd. Qingdao, Shandong, China). All solvents and reagents were of analytical reagent grade or chemically pure, and the solvents were dried in advance and distilled before use.

A Bruker Vector 22 Fourier Transform Infrared (FTIR) Spectrometer was utilized to record the infrared spectra of ackee oil samples. The FTIR spectrum was recorded between 4000 and 500 cm⁻¹. The spectrum was obtained by averaging 20 scans recorded at a resolution of 2 cm⁻¹. Spectra were baseline-corrected. OPUS software was used to acquire and manipulate the spectral data.