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Sk n fi

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The SK-N-FI is a neuroblastoma cell line derived from a metastatic tumor of the human nervous system. It is a commonly used model for the study of neuroblastoma, a type of cancer that originates in the nerve tissue. The SK-N-FI cell line maintains the characteristics of the original tumor and is a valuable tool for researchers investigating the biology and treatment of this disease.

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Lab products found in correlation

Imr 5 75, by Leibniz Institute DSMZ (4 mentions) Lan 6, by Leibniz Institute DSMZ (2 mentions) 1640 media, by Thermo Fisher Scientific (2 mentions) Plasmotest kit, by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Sk n be 2 cells, by American Type Culture Collection (2 mentions)

2 protocols using sk n fi

1

Neuroblastoma Cell Line Authentication

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Neuroblastoma cell lines LAN-6, GI-ME-N, as well as SK-N-FI were directly purchased from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ, Braunschweig, Germany) or American Type Culture Collection (ATCC/LGC Standards, Molsheim Cedex, France), respectively. Furthermore, SK-N-BE(2)C, IMR-5/75, and CLB-GA were provided by the laboratories of H. Deubzer, F. Westermann and J. H. Schulte, respectively. All cell lines not directly purchased from ATCC or DSMZ were authenticated by STR profiling at the DSMZ. IMR-5/75, SK-N-BE(2)C, GI-ME-N, and CLB-GA were grown in RPMI1640 with 10% FBS; LAN-6 and SK-N-FI were cultured in DMEM with 20% FBS. All cell lines were cultured without antibiotics and routinely tested negative for mycoplasma.
Peifer M., Hertwig F., Roels F., Dreidax D., Gartlgruber M., Menon R., Krämer A., Roncaioli J.L., Sand F., Heuckmann J.M., Ikram F., Schmidt R., Ackermann S., Engesser A., Kahlert Y., Vogel W., Altmüller J., Nürnberg P., Thierry-Mieg J., Thierry-Mieg D., Mariappan A., Heynck S., Mariotti E., Henrich K.O., Glöckner C., Bosco G., Leuschner I., Schweiger M.R., Savelyeva L., Watkins S.C., Shao C., Bell E., Höfer T., Achter V., Lang U., Theissen J., Volland R., Saadati M., Eggert A., de Wilde B., Berthold F., Peng Z., Zhao C., Shi L., Ortmann M., Büttner R., Perner S., Hero B., Schramm A., Schulte J.H., Herrmann C., O’Sullivan R.J., Westermann F., Thomas R.K, & Fischer M. (2015). Telomerase activation by genomic rearrangements in high-risk neuroblastoma. Nature, 526(7575), 700-704.
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2

Neuroblastoma Cell Lines Culture Protocol

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The human neuroblastoma cells lines, NB69, SK-N-FI and IMR-5/75, were purchased from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (Braunschweig, Germany) and cultured in Roswell Park Memorial Institute (RPMI) 1640 media (GIBCO) supplemented with 10% fetal calf serum. SK-N-BE(2) cells were purchased from the American Type Culture Collection (Wesel, Germany), and cultured in Dulbecco’s modified Eagle’s medium (DMEM, GIBCO) supplemented with 10% fetal calf serum. The NXS2 murine neuroblastoma cell line19 (link) was kindly provided by Holger N. Lode (University Medicine Greifswald, Germany) and cultured in DMEM supplemented with 10% fetal calf serum, 100 U/mL penicillin and 100 µg/mL streptomycin. All cell lines were cultured at 37°C in 5% CO2. Cell line identity was assured by short tandem repeat DNA genotyping. Cultures were routinely tested for mycoplasma using the PlasmoTest Kit (Invitrogen).
Zirngibl F., Ivasko S.M., Grunewald L., Klaus A., Schwiebert S., Ruf P., Lindhofer H., Astrahantseff K., Andersch L., Schulte J.H., Lode H.N., Eggert A., Anders K., Hundsdoerfer P, & Künkele A. (2021). GD2-directed bispecific trifunctional antibody outperforms dinutuximab beta in a murine model for aggressive metastasized neuroblastoma. Journal for Immunotherapy of Cancer, 9(7), e002923.
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