Slides with transverse tissue sections were selected from the central portion of the scaffolds for staining with the Masson trichrome kit (ThermoFisher). Acquired images were analyzed using the Neurolucida software (MBF Bioscience, Williston, Vermont, USA). A digital grid with 100 μm divisions was overlaid on the tissue, and digital markers for collagen scar or unscarred tissue were placed at each grid intersection within the scaffold channels. The area of collagen scar and unscarred tissue within scaffold channels was then calculated using the Cavalieri probe in the Stereo Investigator software (MBF Bioscience), as previously described (Hakim et al. 2015 (link))
Masson trichrome kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Masson trichrome kit is a histological staining kit used to differentiate between different types of tissue structures. It stains collagen fibers blue, muscle fibers red, and nuclei black. The kit provides the necessary reagents and instructions to perform the Masson trichrome staining procedure.
Automatically generated - may contain errors
Lab products found in correlation
Stereo investigator software, by MBF Biosciences (1 mentions)
Neurolucida software, by MBF Biosciences (1 mentions)
Picrosirius red kit, by Polysciences (1 mentions)
Mtt assay kit, by Cayman Chemical (1 mentions)
Sodium hydrogen sulfide nahs, by Merck Group (1 mentions)
Enos antibody, by BD (1 mentions)
Permount, by Thermo Fisher Scientific (1 mentions)
5 protocols using masson trichrome kit
1
Quantifying Collagen Scar in Tissue Scaffolds
2
Quantifying Cardiac Fibrosis and Apoptosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The explanted hearts were collected on day 28 post-treatment. The tissue was fixed with 4% paraformaldehyde at 4°C for 4 h and then immersed in 30% sucrose at 4°C overnight. Ten-micrometer-thick serial cryosections were obtained, and every 30th section was selected and permeabilized with 0.2% Triton X-100 for 10 min at room temperature. Masson Trichrome Kit and Tunel Cell Apoptosis Detection Kit were purchased from Thermo Fisher Scientific (Cat: 87019) and Servicebio (G1507-20T), respectively. The staining was performed according to the manufacturer’s recommendations. The volume fraction of interstitial collagen was calculated as the ratio of the fibrotic area to the total surface area of the left ventricle. Intramural vessels, perivascular collagen, endocardium, and trabeculae were excluded from this particular analysis. The apoptotic cells were quantified as the number of TUNEL-positive cells divided by the total number of cells and expressed as a percentage (six views per slice and five slices per heart were analyzed).
3
Kidney Collagen Histology Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Formalin fixed, paraffin embedded kidney sections (5 µm thickness) were stained for collagen using Masson Trichrome kit (Thermo Scientific) and Picrosirius red kit (Polysciences, Warrington, PA) following manufacturer’s instructions. Images were captured using Olympus Fluoview1000 microscope (B&B Microscope Ltd, PA).
4
Cardiac Remodeling Signaling Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sodium hydrogen sulfide (NaHS) was purchased from Sigma Aldrich (St. Louis, MO), eNOS antibody from BD Biosciences (Cat. no.: 610297, San Jose, CA), anti-homocysteine (Cat. no.: AB15154), caveolin-1 (Cat. no.: PA5–37506), connexin-40 (Cat. no.: AB38580) and Connexin-43 (Cat. no.: Ab11370) and Ki-67 (AB15580) antibodies from Abcam (Cambridge, MA), BrdU, MMP-2-9 and -13 (Cat. nos.: MAB4072, AB19167, AB19016 and AB8120 respectively) from Millipore (Burlington, MA), TIMP-1, -2, -4 and GAPDH (Cat. nos.: SC5538, SC6835, SC9375 and SC47724 resp.) and secondary antibodies for mouse and rabbit (SC358920 and SC2004 resp.) from Santa Cruz Biotechnology (Dallas, TX), Masson Trichrome kit from Fisher Scientific, USA, Picrosirius from Polysciences Inc, (Warrington, PA) and MTT assay kit from Cayman Chemical (Ann Arbor, MI).
5
Masson Trichrome Staining of Paraffin Biopsies
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Paraffin-embedded biopsies sectioned at 4 microns were stained with a Masson Trichrome Kit according to manufacturer’s protocol (Fisher Scientific Richard Allan Scientific #22-110-648, Pittsburg, PA, USA). Briefly, slide specimens were deparaffinized in xylene, hydrated and then incubated at room temperature in Bouin’s fixative for 16 h. Nuclei were stained with Weigert’s Hematoxylin, myofiber cytoplasm with Scarlet Red, and collagen with Aniline Blue dye, with washes in-between steps. Stained slide specimens were dehydrated and mounted with Permount (Fisher Scientific #SP15-100, Pittsburg, PA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!