Nbp1 93 613

LRRC6 and DNAH5 antibodies were previously described [18 (link), 19 (link)]. Antibodies against REPTIN (ab89942; Abcam, Cambridge, UK), IQUB (HPA020621; Sigma-Aldrich, MO, USA), TCTEX1D1 (HPA028420; Sigma-Aldrich, MO, USA), DYX1C1 (info. Omitted), C21ORF59 (sc-365792; Santa Cruz Biotechnology, TX, USA), DNAI1 (SAB4501181; Novus Biologicals, Littleton, CO, USA), DNAI2 (H00064446-M01; Abnova, Taipei, Taiwan), DNAH7 (NBP1-93,613; Novus Biologicals, Littleton, CO, USA), acetylated α-tubulin (T7451 from Sigma-Aldrich and 5335S from Cell Signaling Technology, Danvers, MA, USA), FLAG (#8146; Cell Signaling Technology, Danvers, MA, USA), Myc (#2276; Cell Signaling Technology, Danvers, MA, USA), FOXJ1 (sc-53139; Santa Cruz Biotechnology, TX, USA), KPNB1(nb100-79,806; Novus Biologicals, Littleton, CO, USA), and β-actin (sc-1615; Santa Cruz Biotechnology, TX, USA) were purchased from commercial sources. Secondary antibodies were purchased from Invitrogen and Santa Cruz Biotechnology for immunofluorescence and immunoblotting analyses.

A polyclonal antibody recognizing the C-terminal sequence (amino acids 339–362, DRLERENKGKWQAIAKHQLQHVFS) of mouse ZMYND10 was recovered from rabbits injected with the corresponding antigen (AbFrontier, Seoul, Korea). LRRC6 and DNAH5 antibodies were previously described [37 (link), 38 (link)]. Antibodies against DNAI2 (H00064446-M01; Abnova, Taipei, Taiwan); REPTIN (ab89942; Abcam, Cambridge, UK); DNAH7 (NBP1-93613) and DNAI1 (SAB4501181; both from Novus Biologicals, Littleton, CO, USA); IQUB (HPA020621) and TCTEX1D1 (HPA028420; both from Sigma-Aldrich); acetylated α-tubulin (T7451 from Sigma-Aldrich and 5335S from Cell Signaling Technology, Danvers, MA, USA); FLAG (#8146) and Myc (#2276; both from Cell Signaling Technology); and C21ORF59 (sc-365792) and β-actin (sc-1615; both from Santa Cruz Biotechnology) were purchased from commercial sources. Secondary antibodies were purchased from Invitrogen and Santa Cruz Biotechnology for immunofluorescence and immunoblotting analyses, respectively.