The following antibodies were used for western blots: from Cell Signaling, Danvers, MA: total mTOR (1:1000; catalog number 2983S), total S6K1 (1:1000; catalog number 9202S), pS6K1 Thr389 (1:1000; catalog number 9205S), β-actin (1:10000; catalog number 3700S), total IRS-1 (1:1000; catalog number 3407S), IRS Ser318 (1:1000; catalog number 5610S), IRS Ser636/639 (1:1000; catalog number 2388S), totalPI3K p85 (1:1000; catalog number 4292S), PI3K Tyr458 (1:1000; catalog number 4228S), total AKT (1:1000 ; catalog number 9272), AKT Thr308 (1:1000; catalog number 2965S), AKT Ser473 (1:1000; catalog number3787S) total GSK3β (1:1000; catalog number 9315S), GSK3β Ser9 (1:1000; catalog number 9336S), CP13 (1:1000; catalog number 11834S). From Millipore, Billerica, MA: APP C-terminal (1:2000; catalog number 171610), Tau5 (1:5000; catalog number 577801). From BioLegend, San Diego, CA: APP (1:3000; catalog number MAB348). PHF-1 (1:3000) was a gift from Dr. Peter. Davies.
Totalpi3k p85
Manufactured by Cell Signaling Technology
Sourced in United States
The TotalPI3K p85 is a protein quantification assay kit produced by Cell Signaling Technology. The kit is designed to detect and quantify the p85 regulatory subunit of phosphoinositide 3-kinase (PI3K) in cell and tissue lysates.
Automatically generated - may contain errors
Lab products found in correlation
Total akt, by Cell Signaling Technology (2 mentions)
β actin, by Cell Signaling Technology (2 mentions)
Total gsk3β, by Cell Signaling Technology (1 mentions)
Gsk3β ser9, by Cell Signaling Technology (1 mentions)
Total s6k1, by Cell Signaling Technology (1 mentions)
App c terminal, by Merck Group (1 mentions)
Akt thr 308, by Cell Signaling Technology (1 mentions)
Total irs 1, by Cell Signaling Technology (1 mentions)
Akt ser473, by Cell Signaling Technology (1 mentions)
P s6k1 thr389, by Cell Signaling Technology (1 mentions)
Total mtor, by Cell Signaling Technology (1 mentions)
Human fibrinogen, by Merck Group (1 mentions)
Human thrombin, by Merck Group (1 mentions)
Fibrinogen, by Merck Group (1 mentions)
Phospho plcγ2, by Cell Signaling Technology (1 mentions)
Total syk, by Cell Signaling Technology (1 mentions)
Phospho akt at ser473, by Cell Signaling Technology (1 mentions)
Total erk, by Cell Signaling Technology (1 mentions)
Rhodamine phalloidin, by Merck Group (1 mentions)
Phycoerythrin pe conjugated isotype control iggs, by Emfret Analytics (1 mentions)
2 protocols using totalpi3k p85
1
Western Blot Antibody Validation Protocol
2
Platelet Signaling Pathway Analysis
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Human thrombin, PGE1, rhodamine-phalloidin, dimethyl sulfoxide (DMSO), ADP, fibrinogen, human fibrinogen, ferric chloride (FeCl3), Acetylsalicylic acid (ASA), carboxymethylcellulose (CMC), and all the reagents were purchased from Sigma (St. Louis, MO, USA). Equine tendon collagen (type I) and ATP luciferin/luciferase reagent were obtained from Chrono-log (Havertown, PA, USA). CRP was obtained from Dr Richard Farndale (Department of Biochemistry, University of Cambridge, UK). Phycoerythrin (PE)-conjugated isotype control IgGs, rat monoclonal antibodies against mouse P-selectin, activated αIIbβ3 (JON/A) were from Emfret Analytics (Eibelstadt, Germany). Antibodies against phospho-Syk at Tyr525/526 (Cat# 2710S), phospho-PLCγ2 at Tyr1217 (Cat# 3871S), phospho-PI3K p85α/β at Tyr458/p55α/γ at Tyr199 (Cat# 4228S), phospho-Akt at Ser473 (Cat# 9271S), phospho-ERK at Thr202/Tyr204 (Cat# 4337S), Total Syk (Cat# 12358S), Total PLCγ2 (Cat# 3872S), Total PI3K p85 (Cat# 4249S), Total Akt (Cat# 9272S), Total ERK (Cat# 9102S), and β-actin (Cat# 4967S) were obtained from Cell Signaling (Danvers, MA, USA). Monoclonal antibodies against phospho-integrin β3 at Tyr 759 and Total integrin β3 were obtained from Santa Cruz (Santa Cruz, CA, USA). Calcium dye (FLIPR Calcium Assay kit) was from Molecular Devices (Sunnyvale, CA, USA).
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