Malvern Z90 Zetasizer equipped with a 633 nm laser and an avalanche photodiode detector was used to characterize the hydrodynamic size. 1H NMR spectra were recorded using a Bruker 400 NMR spectrometer. Transmission Electron Microscopy (TEM) images were recorded on a FEI Tecnai 20 (type Sphera). Scanning electron microscopy (SEM) images were characterized by FEI Quanta 200 3D FEG. Ultraviolet-visible Spectroscopy (UV/Vis) absorbance was recorded on a Jasco V-650 UV/Vis spectrometer. The O2 concentration was determined using a portable meter MultiLine® Multi 3510 IDS. Microplate reader (Safire2, TECAN) was employed for the CCK-8 assay. Fluorescence images were observed and captured by QuantaMaster-40 fluorescence S-3 spectrophotometer and Leica TCS SP5X. A Becton Dickinson FACScan flow cytometer was used to determine the cellular uptake. The photoacoustic images were captured by an in vivo photoacoustic system (MSOT inVision, iThera Medical). The in vivo circulation behavior was recorded by an in vivo imaging system (PerkinElmer IVIS Lumina Series III). The ultrasound imaging was performed on a Mindray resona 7. FUS treatment was conducted using Intelect® Mobile Ultrasound. Tumor position temperature changes were collected by a thermal imager (FLIR A300, IRS Systems Inc.) coupled with an infrared camera.
Quanta 200 3d feg
Manufactured by Thermo Fisher Scientific
The Quanta 200 3D FEG is a scanning electron microscope (SEM) designed for high-resolution imaging and microanalysis. It features a field emission gun (FEG) electron source, providing high-quality images with enhanced resolution and surface detail. The instrument is capable of operating in various imaging modes, including secondary electron, backscattered electron, and low-voltage imaging, to suit a wide range of sample types and applications.
Automatically generated - may contain errors
Lab products found in correlation
Safire2, by Tecan (2 mentions)
Tecnai 20, by Thermo Fisher Scientific (1 mentions)
Facscan flow cytometer, by BD (1 mentions)
Tcs sp5x, by Leica (1 mentions)
V 650 uv vis spectrometer, by Jasco (1 mentions)
Ivis lumina series 3, by PerkinElmer (1 mentions)
Resona 7, by Mindray (1 mentions)
Tcs sp8 x, by Leica (1 mentions)
Tu e cryotitan, by Thermo Fisher Scientific (1 mentions)
Prominence 1 gpc system, by Shimadzu (1 mentions)
2 protocols using quanta 200 3d feg
1
Multimodal Characterization of Nanoparticles
2
Comprehensive Nanoparticle Characterization Protocol
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The copolymers were analyzed with a Bruker AV 400 MHz Ultra-shieldTM spectrometer and Prominence-I GPC system (Shimadzu) with a PL gel 5 μm mixed D (Polymer Laboratories), equipped with a RID-20A differential refractive index detector. The hydrodynamic size and dispersity index (PDI) of the nanoparticles were determined by a Malvern instruments Zetasizer (model Nano ZSP) dynamic light scattering (DLS) equipped with a 633 nm He-Ne laser and avalanche photodiode detector. The morphologies of the formed nanoparticles were recorded with a FEI Quanta 200 3D FEG scanning electron microscopy (SEM). Cryogenic transmission electron microscopy (cryo-TEM) and cryo-electron tomography (cryo-ET) experiments were conducted on the TU/e CryoTITAN (Thermo Fisher Scientific) equipped with a fieldemission gun operating at 300 kV, an autoloader station and a post-column Gatan energy filter. Fluorescent images were recorded using a Confocal Laser Scanning Microscopy (CLSM, Leica TCS SP8X) equipped with two-photon laser source (Chameleon Vision, Coherent, USA). Cell viability was evaluated via a microplate reader (Safire2, TECAN). Nanosight Tracking Analysis was performed on a Nanosight NS300 equipped with a laser channel (488 nm) and sCMOS camera.
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