Poly(styrene-co-maleic anhydride) (an SMA copolymer), with a mean molecular weight of 1600 Da, and mTHPC were purchased from Sigma Chemical Co. (St. Louis, MO, USA). 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (WSC) were purchased from Wako Pure Chemical Industries Ltd. (Osaka, Japan). 2,2,6,6-Tetramethyl-4-piperidone (4-oxo-TEMP) was purchased from Tokyo Chemical Industry (Tokyo, Japan). Other reagents of reagent grade and solvents were purchased from Wako Pure Chemical Industries Ltd. and used without further purification.
3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide mtt
Manufactured by Fujifilm
Sourced in Japan
3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) is a chemical compound used as a colorimetric assay for measuring the activity of enzymes that reduce metabolic dyes, such as the NAD(P)H-dependent cellular oxidoreductase enzymes. It provides a quantitative measure of viable cells.
Automatically generated - may contain errors
Lab products found in correlation
Poly styrene co maleic anhydride, by Merck Group (1 mentions)
2 2 6 6 tetramethyl 4 piperidone 4 oxo temp, by Tokyo Chemical Industry (1 mentions)
Mthpc, by Merck Group (1 mentions)
Nifedipine, by Fujifilm (1 mentions)
Hydrogen peroxide h2o2, by Fujifilm (1 mentions)
Anti icam 1 antibody, by Santa Cruz Biotechnology (1 mentions)
Magnesium chloride hexahydrate, by Fujifilm (1 mentions)
Zinc chloride, by Fujifilm (1 mentions)
Methanol, by Fujifilm (1 mentions)
Dimethyl sulfoxide (dmso), by Fujifilm (1 mentions)
Amplitaq gold 360 master mix, by Thermo Fisher Scientific (1 mentions)
Human free bdnf elisa kit, by R&D Systems (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Rnalater, by Thermo Fisher Scientific (1 mentions)
Revertra ace, by Toyobo (1 mentions)
3 protocols using 3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2h tetrazolium bromide mtt
1
Synthesis and Characterization of SMA-mTHPC Nanoparticles
2
Nifedipine-Induced Oxidative Stress Assay
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Nifedipine [1,4-dihydro-2,6-dimethyl-4-(2-nitrophenyl)-3,5-pyridinedicarboxylic acid dimethyl ester], hydrogen peroxide (H2O2), and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) were purchased from Wako (Osaka, Japan). Dihydroethidium (DHE) was purchased from DOJINDO (Kumamoto, Japan). The anti-ICAM-1 antibody was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). The anti-mouse/rat AGT antibody was obtained from Immuno-Biological Laboratories (Takasaki, Japan).
3
Optimizing Cell Culture Conditions with DMEM, RNAlater, and TRIzol
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Dulbecco s modified Eagle s medium (DMEM) , RNAlater, and TRIzol were obtained from Life Technologies (Carlsbad, CA, USA) , and ReverTra Ace was purchased from Toyobo (Osaka, Japan) . AmpliTaq Gold 360 Master Mix was obtained from Applied Biosystems (Foster City, CA, USA) . The Human Free BDNF ELISA Kit was purchased from R&D Systems (Minneapolis, MN, USA) . The primers used in this study (Table 1) were obtained from GeneNet (Fukuoka, Japan) or Greiner Japan (Kanagawa, Japan) . Whole-wheat flour and wheat endosperm were purchased from Hara Farm (Kumamoto, Japan) , and wheat bran was obtained from YouTech (Hokkaido, Japan) . Magnesium chloride hexahydrate, zinc chloride, methanol, 3-(4,5-dimethyl-2-thiazolyl) -2,5-diphenyl-2H-tetrazolium bromide (MTT) , dimethyl sulfoxide (DMSO) , and other reagents were obtained from Wako Pure Chemical Industry (Osaka, Japan) .
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