Immunofluorescent cytochemical staining for STAT3 was performed. Cells were grown in chamber slides with varying concentration of LB100 for 4 hours. Cells were fixed with 2% paraformaldehyde, washed with PBS, permeabilized with 1% Triton X-100, washed again with PBS, and blocked with 1% BSA. Rabbit anti-STAT3 (D3Z2G) antibody (Cell signaling) was added at 1:1000 dilution and incubated for 1.5 hours at room temperature. Cells were washed with 1% BSA and mouse anti-rabbit-FITC antibody (Jackson ImmunoResearch) was added at 1:100 and incubated for 1 hour at room temperature. Nuclei were counterstained with DAPI (Sigma-Aldrich). Coverslips were mounted with VectaShield anti-fade solution (Vector Labs) and slides examined on a Nikon Eclipse CI fluorescent microscope and imaged with a QImaging camera.
Rabbit anti stat3 d3z2g antibody
Manufactured by Cell Signaling Technology
Sourced in United States
The Rabbit anti-STAT3 (D3Z2G) antibody is a research-use only product from Cell Signaling Technology. It is a primary antibody that specifically recognizes the STAT3 protein. STAT3 is a transcription factor that plays a role in cellular processes such as cell growth and survival.
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Lab products found in correlation
2 protocols using rabbit anti stat3 d3z2g antibody
1
Immunofluorescent Analysis of STAT3
2
Immunoblotting of Cell Signaling Proteins
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Rabbit anti-NKX6.1 antibody (1:500; Sigma, HPA036774, St. Louis, MO, USA), rabbit anti-phospho-STAT3 Tyr705 (D3A7) antibody (1:1000; Cell Signaling technology, X9145, Danvers, MA, USA), rabbit anti-STAT3 (D3Z2G) antibody (1:1000; Cell Signaling Technology, 12640), rabbit anti-IL-6 antibody (1:1000; Cell Signaling Technology, 12153), rabbit anti-FLAG antibody (1:500; Sigma, F7425) and mouse anti-beta actin antibody (1:5000; Abcam, ab6276) were used for primary reactions. The Easy-Western-II detection system (Beacle, BCL-EZS21, Kyoto, Japan) was used instead of secondary antibody for rabbit IgG. We used goat anti-mouse IgG antibody conjugated to a peroxidase (1:50,000; Pierce biotechnology, 31340, Rochford, IL, USA) to detect beta-actin signals. Images were obtained with Ez-Capture II (ATTO, Tokyo, Japan) and ImageSaver5 Software (ATTO).
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