Mouse 3T3-L1 preadipocytes were obtained from American Type Culture Collection (Manassas, VA). 3T3-L1 preadipocyte medium, 3T3-L1 adipocyte differentiation medium, and 3T3-L1 adipocyte maintenance medium were obtained from Zen-Bio, Inc. (Research Triangle Park, NC). [1, 2-3H] 2-deoxy-D-glucose was purchased from Perkin Elmer Life Sciences (Waltham, MA). Hypochlorous sodium solution, human insulin, Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME), SP600125, and antibody against phospho-IRS-1 (Tyr612) were purchased from Sigma-Aldrich (St. Louis, MO). Protein A/G-agarose, RIPA lysis buffer, PS-1145, IKKβ siRNA, JNK2 siRNA, PKCθ siRNA, control siRNA, and antibodies against β-actin, GAPDH and Na+/K+ ATPase were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Antibodies against phospho-IRS-1 (Ser307), IRS-1, phospho-Akt (Ser473), phospho-Akt (Thr308), Akt, phospho-GSK3β (Ser9), GSK3β, phospho-IKKα/β (Ser176/180), IKKα, IKKβ, phospho-SAPK/JNK (Thr183/Tyr185), JNK, phospho-PKCθ (Thr538), PKCθ, GLUT4, IκBα, and horseradish peroxidase-linked secondary antibodies (Beverly, MA). ONOO− was obtained from Calbiochem (Billerica, MA). All other chemicals were of the highest commercial grade available.
Jnk2 sirna
Manufactured by Santa Cruz Biotechnology
Sourced in United States
JNK2 siRNA is a small interfering RNA (siRNA) targeting the JNK2 gene, which is involved in various cellular processes. This product is designed to reduce the expression of the JNK2 protein in cells, allowing researchers to study its biological functions.
Automatically generated - may contain errors
Lab products found in correlation
Control sirna, by Santa Cruz Biotechnology (3 mentions)
Human insulin, by Merck Group (2 mentions)
Pkc θ sirna, by Santa Cruz Biotechnology (2 mentions)
Ikkβ sirna, by Santa Cruz Biotechnology (2 mentions)
Hypochlorous sodium solution, by Merck Group (2 mentions)
Antibody against phospho irs 1 tyr612, by Merck Group (2 mentions)
Ps 1145, by Santa Cruz Biotechnology (2 mentions)
Protein a g agarose, by Santa Cruz Biotechnology (2 mentions)
Ripa lysis buffer, by Santa Cruz Biotechnology (2 mentions)
Mouse 3t3 l1 preadipocytes, by American Type Culture Collection (2 mentions)
Anisomycin, by Merck Group (1 mentions)
Jnk1 sirna, by Santa Cruz Biotechnology (1 mentions)
Pkcα sirna, by Santa Cruz Biotechnology (1 mentions)
Tapi 0, by Merck Group (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
α amanitin, by Merck Group (1 mentions)
Actinomycin d, by Merck Group (1 mentions)
Cycloheximide (chx), by Merck Group (1 mentions)
Phorbol myristate acetate (pma), by Merck Group (1 mentions)
Sp600125, by Merck Group (1 mentions)
3 protocols using jnk2 sirna
1
Insulin Signaling in 3T3-L1 Adipocytes
2
Modulation of TACE Activation by PKCβ
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were maintained in DMEM with 10% FBS, 100 units/ml penicillin, and 100 μg/ml streptomycin. For drug treatments, 90% confluent cells were serum-starved for 4 h and then treated with 0.5 μm PMA (Sigma) or SP600125 (Merck Millipore, Billerica, MA). A PKCβ-selective inhibitor (539654, Merck Millipore), anisomycin (Sigma), and TAPI-0 (Merck Millipore) were used for 30 min before drug treatment. Cycloheximide, α-amanitin, and actinomycin D were purchased from Sigma. For overexpression or knockdown of specific proteins, transfection of cDNA or siRNA was performed. When cells reached 30%–40% confluence, cells were transfected with JNK1 siRNA, JNK2 siRNA, PKCα siRNA, PKCβ siRNA, and TACE siRNA (Santa Cruz Biotechnology, Dallas, TX), WT PKCβII cDNA, DN PKCβI (K371R), and DN PKCβII (K371R) cDNA (Addgene, Cambridge, MA) using Lipofectamine 2000 (Invitrogen) for 48–72 h. Control siRNA (catalog no. sc-37007, Santa Cruz Biotechnology), designed not to target any gene, was used as a negative control in knockdown experiments.
3
Insulin Resistance in 3T3-L1 Adipocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mouse 3T3-L1 preadipocytes were obtained from American Type Culture Collection (Manassas, VA, USA). 3T3-L1 preadipocyte medium, 3T3-L1 adipocyte differentiation medium, and 3T3-L1 adipocyte maintenance medium were obtained from Zen-Bio, Inc. (Research Triangle Park, NC, USA). [1,2-3H] 2-deoxy-d -glucose was purchased from Perkin Elmer Life Sciences (Waltham, MA, USA). Hypochlorous sodium solution, human insulin, Nω-nitro-l -arginine methyl ester hydrochloride (l -NAME), SP600125, and antibody against phospho-IRS1 (Tyr612) were purchased from Sigma–Aldrich. Protein A/G-agarose, RIPA lysis buffer, PS-1145, IKKβ siRNA, JNK2 siRNA, PKCθ siRNA, control siRNA, and antibodies against β-actin, GAPDH, and Na+/K+ ATPase were obtained from Santa Cruz Biotechnology, Inc. Antibodies against phospho-IRS1 (Ser307), IRS1, phospho-Akt (Ser473), phospho-Akt (Thr308), Akt, phospho-GSK3β (Ser9), GSK3β, phospho-IKKα/β (Ser176/180), IKKα, IKKβ, phospho-SAPK/JNK (Thr183/Tyr185), JNK, phospho-PKCθ (Thr538), PKCθ, GLUT4, IκBα, and HRP-linked secondary antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). ONOO− was obtained from Calbiochem (Billerica, MA, USA). All other chemicals were of the highest commercial grade available.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!