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Lds electrophoresis loading buffer

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LDS electrophoresis loading buffer is a solution used in protein electrophoresis to prepare samples for analysis. It denatures and solubilizes proteins, allowing for their separation and detection on a polyacrylamide gel.

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Lab products found in correlation

Protease and phosphatase inhibitor cocktail, by Merck Group (2 mentions) Goat anti actin sc 1616, by Santa Cruz Biotechnology (2 mentions) Irdye conjugated antibody, by LI COR (2 mentions) Sds page gel, by Thermo Fisher Scientific (2 mentions) Abs45, by Merck Group (2 mentions) 660 nm protein assay reagent, by Thermo Fisher Scientific (2 mentions) Odyssey imager, by LI COR (2 mentions) M per lysis buffer, by Thermo Fisher Scientific (2 mentions)

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LDS buffer (121 citations) Loading buffer (104 citations) LDS loading buffer (95 citations)

2 protocols using lds electrophoresis loading buffer

1

Quantitative Analysis of MYPT1 and RhoA Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
NIH/3T3 cells were rinsed once with PBS and lysed in M-PER lysis buffer (Thermo Fisher) supplemented with protease and phosphatase inhibitor cocktail (Sigma). Protein concentration of the lysates cleared of insoluble cell debris were determined using 660 nm Protein Assay reagent (Thermo Fisher). A total of 15 μg of proteins in LDS electrophoresis loading buffer (Life Technologies) was denatured for 10 min at 70 °C and separated on 4–12% SDS-PAGE gel (Life Technologies). Proteins were transferred onto 0.2-μm nitrocellulose membrane (Pall) and processed for Western blotting. Primary antibodies were used at the following dilutions: goat anti-actin (sc-1616, Santa-Cruz Biotechnology, 1:4,000), mouse anti-MYPT1 (612165, Becton-Dickinson, 1:4,000), rabbit anti-MYPT1(pT696) (ABS45, Millipore, 1:500), rabbit anti-RhoA (67B9, Cell Signaling, 1:4,000), rabbit anti-MLC2 (8505, Cell Signaling, 1:4,000), and mouse anti-MLC2(pT19) (3674, Cell Signaling, 1:500). Appropriate secondary IRDye-conjugated antibodies (LI-COR) were used at 1:10,000. Proteins were detected using Odyssey imager (LI-COR). The investigator carrying out the Western blot experiments was not blinded to the identity of the samples.
Vabres P., Sorlin A., Kholmanskikh S.S., Demeer B., St-Onge J., Duffourd Y., Kuentz P., Courcet J.B., Carmignac V., Garret P., Bessis D., Boute O., Bron A., Captier G., Carmi E., Devauchelle B., Geneviève D., Gondry-Jouet C., Guibaud L., Lafon A., Mathieu-Dramard M., Thevenon J., Dobyns W.B., Bernard G., Polubothu S., Faravelli F., Kinsler V.A., Thauvin C., Faivre L., Ross M.E, & Rivière J.B. (2019). Postzygotic inactivating mutations of RHOA cause a mosaic neuroectodermal syndrome. Nature genetics, 51(10), 1438-1441.
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2

Quantitative Analysis of MYPT1 and RhoA Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
NIH/3T3 cells were rinsed once with PBS and lysed in M-PER lysis buffer (Thermo Fisher) supplemented with protease and phosphatase inhibitor cocktail (Sigma). Protein concentration of the lysates cleared of insoluble cell debris were determined using 660 nm Protein Assay reagent (Thermo Fisher). A total of 15 μg of proteins in LDS electrophoresis loading buffer (Life Technologies) was denatured for 10 min at 70 °C and separated on 4–12% SDS-PAGE gel (Life Technologies). Proteins were transferred onto 0.2-μm nitrocellulose membrane (Pall) and processed for Western blotting. Primary antibodies were used at the following dilutions: goat anti-actin (sc-1616, Santa-Cruz Biotechnology, 1:4,000), mouse anti-MYPT1 (612165, Becton-Dickinson, 1:4,000), rabbit anti-MYPT1(pT696) (ABS45, Millipore, 1:500), rabbit anti-RhoA (67B9, Cell Signaling, 1:4,000), rabbit anti-MLC2 (8505, Cell Signaling, 1:4,000), and mouse anti-MLC2(pT19) (3674, Cell Signaling, 1:500). Appropriate secondary IRDye-conjugated antibodies (LI-COR) were used at 1:10,000. Proteins were detected using Odyssey imager (LI-COR). The investigator carrying out the Western blot experiments was not blinded to the identity of the samples.
Vabres P., Sorlin A., Kholmanskikh S.S., Demeer B., St-Onge J., Duffourd Y., Kuentz P., Courcet J.B., Carmignac V., Garret P., Bessis D., Boute O., Bron A., Captier G., Carmi E., Devauchelle B., Geneviève D., Gondry-Jouet C., Guibaud L., Lafon A., Mathieu-Dramard M., Thevenon J., Dobyns W.B., Bernard G., Polubothu S., Faravelli F., Kinsler V.A., Thauvin C., Faivre L., Ross M.E, & Rivière J.B. (2019). Postzygotic inactivating mutations of RHOA cause a mosaic neuroectodermal syndrome. Nature genetics, 51(10), 1438-1441.
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