Ptlink apparatus

Manufactured by Agilent Technologies

The PTLink apparatus is a laboratory instrument designed for the measurement and analysis of various physical and chemical properties. It is a core component used in scientific research and testing applications.

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Lab products found in correlation

Autostainer device, by Agilent Technologies (1 mentions) High ph target retrieval solution, by Agilent Technologies (1 mentions) Envisiontmflex system, by Agilent Technologies (1 mentions)

2 protocols using ptlink apparatus

Detailed Spry1 Gene Expression Analysis by In Situ Hybridization and Phospho-ERK Immunohistochemistry

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Specimens were fixed in 4% paraformaldehyde overnight at four degrees, dehydrated and included in paraffin. Microtome sections were dewaxed and rehydrated using a xylene/ethanol gradient. For in situ hybridization, the RNAscope^® Intro Pack 2.5 HD Reagent Kit Brown- Mm was used exactly as directed by the manufacturers. Probe used was Mm-Spry1 (Cat # 491,311), which targets sequences within the third exon. This is the only coding exon of mouse Spry1 gene and is completely removed in Spry1 knockout mice. For phospho-ERK immunohistochemistry, samples were subjected to antigen retrieval (95 °C for 20 min in Tris/EDTA buffer, pH 9) using a PTLink apparatus (DAKO). Staining was performed by an Autostainer device (DAKO), using anti-phospho-p44/42 MAPK (D13.14.4E) XP® Rabbit mAb from Cell Signaling Technologies at 1/200 dilution. Automated Hematoxylin–Eosin staining was performed using a Coverstainer device (DAKO).

Altés G., Vaquero M., Cuesta S., Anerillas C., Macià A., Espinet C., Ribera J., Bellusci S., Klein O.D., Yeramian A., Dolcet X., Egea J, & Encinas M. (2022). A dominant negative mutation uncovers cooperative control of caudal Wolffian duct development by Sprouty genes. Cellular and Molecular Life Sciences, 79(10), 514.

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Paraffin-Embedded Tissue Immunohistochemistry

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Tissue material was fixed in 10% buffered formalin at pH 7.4 and placed in a processor. The tissue was embedded in paraffin at 60°C using standard histopathological methods. The marked paraffin blocks were sliced using a microtome into 4-5-μm thick sections. The sections were then fixed to microscope slides and left for 1 h at 60°C. The Dako EnVision TM FLEX + system (Dako, Santa Clara, USA) and the immunohistochemical method were used. High pH Target Retrieval Solution (Dako) was used as the buffer in the PT-link apparatus (Dako) at a temperature of 97°C for 20 min.

Markowska A., Gryboś A., Marszałek A., Bednarek W., Filas V., Gryboś M., Markowska J., Mądry R., Więckowska B., Nowalińska D, & Szarszewska M. (2021). Expression of selected molecular factors in two types of endometrial cancer. Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 30(10).

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