1m naoh

Manufactured by Thermo Fisher Scientific

Sourced in France

1M NaOH is a concentrated sodium hydroxide solution with a molarity of 1 mol/L. It is a common laboratory reagent used for various applications, such as pH adjustment, titration, and sample preparation.

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Lab products found in correlation

Opti cho medium, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Corning (1 mentions) Powercho 2 cd, by Lonza (1 mentions) L glutamine, by Corning (1 mentions) Antifoam 204, by Merck Group (1 mentions) Antifoam c, by Merck Group (1 mentions) Procho5, by Lonza (1 mentions) Cd dynamis, by Thermo Fisher Scientific (1 mentions) Antifoam ex cell, by Merck Group (1 mentions) Antifoam se 15, by Merck Group (1 mentions) Antifoam y 30, by Merck Group (1 mentions) D gluconolactone, by Merck Group (1 mentions) Granulated agar, by BD (1 mentions) Sodium hydroxide (naoh), by Thermo Fisher Scientific (1 mentions)

4 protocols using 1m naoh

Optimizing CHO Cell Culture Media

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Chemically-defined (CD) OptiCHO medium (Thermo Fisher Scientific, Waltham, MA) was the original media in which the cell line was propagated. The cells were adapted to four other commercially available media including: CD Dynamis (Thermo Fisher Scientific, Carlsbad, CA), protein-free ProCHO 5 (Lonza, Walkersville, MD), CD PowerCHO 2 (Lonza, Walkersville, MD), and CD EX-Cell Advanced (SAFC, Lenexa, KS). All media studied were supplemented with 8 mM L-glutamine (Corning, Manassas, VA) and 1X Penicillin/Streptomycin (Corning, Oneonta, NY). Initial glucose concentration in each media varied, based on the manufacturer’s proprietary formulation, and ranged between 5 and 8 g/L. ProCHO 5 and EX-Cell Advanced had the highest glucose concentration, while OptiCHO had the lowest glucose concentration. Five commercially available antifoams (Sigma Life Sciences, St. Louis, MO) were studied: Antifoam C, Antifoam EX-Cell, Antifoam 204, Antifoam SE-15 and Antifoam Y-30. Cell culture pH was controlled using 1M NaOH (Thermo Fisher Scientific, Fair Lawn, NJ).

Velugula‐Yellela S.R., Williams A., Trunfio N., Hsu C., Chavez B., Yoon S, & Agarabi C. (2017). Impact of media and antifoam selection on monoclonal antibody production and quality using a high throughput micro‐bioreactor system. Biotechnology Progress, 34(1), 262-270.

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Optimizing Cheese Curd pH Adjustment

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Each batch of SM and concentrates was divided into 2 batches: one was kept at the initial pH (pHi), whereas the second was adjusted to pH 6.50, which corresponds to the usual renneting pH for cheddar cheese (Ong et al., 2012) . For the pH-adjusted batches, the pH was adjusted by adding d-gluconolactone (Sigma-Aldrich, St. Louis, MO) or 1 M NaOH (Thermo Fisher Scientific, Waltham, MA) to reach approximately pH 6.45, and samples were equilibrated overnight at 4°C. Before analyses or coagulation tests, the pH was readjusted, if necessary, with 1 M NaOH to reach 6.50 and samples were warmed to 32°C and held for 1 h at this temperature.

Lauzin A., Bérubé A., Britten M, & Pouliot Y. (2019). Effect of pH adjustment on the composition and rennet-gelation properties of milk concentrates made from ultrafiltration and reverse osmosis. Journal of dairy science, 102(5).

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Cheese-Based Solid Medium Preparation

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A flask containing cheese slurry was pH adjusted to 5.8, 7.0, or 7.5, with 1 M NaOH (Fisher Scientific), followed by the addition of 2 g granulated agar (BD Biosciences, France) and NaCl (0%, 2%, 4%, or 8% (w/v)). The final mixture was pasteurized by heating at 80 °C for 10 min. After cooling down to 45–50 °C, the pour plate technique was applied to prepare the cheese-based (curd) solid medium contained in Petri dishes, which were left to solidify for 2 h before inoculation with A. arilaitensis Po102.

Sutthiwong N., Fouillaud M, & Dufossé L. (2018). The Influence of pH, NaCl, and the Deacidifying Yeasts Debaryomyces hansenii and Kluyveromyces marxianus on the Production of Pigments by the Cheese-Ripening Bacteria Arthrobacter arilaitensis. Foods, 7(11), 190.

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Alkaline Cr(VI) and Tc(VII) Simulation

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In this work a background solution of 1M NaOH (prepared from 95% NaOH pellets, Fisher Scientific), was adjusted to 1560 ppm Cr(VI) with Na 2 Cr 2 O 7 •2H 2 O (≥99.5%, Allied Chemical) to achieve an alkaline pH level and Cr(VI) concentration similar to nuclear waste streams. 28 For experiments requiring Tc(VII), a 10,000 ppm NH 4 TcO 4 stock solution was used to adjust Cr(VI) solutions to within 1 and 1000 ppm Tc(VII). For control experiments, without Cr(VI), Tc(VII) was added directly to 1M NaOH.

Saslow S.A., Um W., Pearce C.I., Engelhard M.H., Bowden M.E., Lukens W., Leavy I.I., Riley B.J., Kim D.S., Schweiger M.J, & Kruger A.A. (2017). Reduction and Simultaneous Removal of ⁹⁹Tc and Cr by Fe(OH)₂(s) Mineral Transformation. Environmental science & technology, 51(15).

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