Brassica napus L. cv. Topas (rapeseed) and Hordeum vulgare L. cv. Igri (barley) were used as donor plants. Barley seeds were germinated in soil for 1 month at 4°C. After that, they were grown at 12°C with a 12/12 light/dark cycle (10,000–16,000 lx) for 1 month in a plant growth chamber (Sanyo; relative humidity about 70%), and then in a greenhouse under a controlled temperature of 18°C. Rapeseed seeds were sown in soil and plants were grown under controlled conditions at 15/10°C in a 16/8 h light/dark cycle in a plant growth chamber (Sanyo) with 60% relative humidity.
Plant growth chamber
Manufactured by Sanyo
Sourced in Japan
The Sanyo plant growth chamber is a controlled environment system designed to simulate various environmental conditions for plant cultivation. It provides precise control over temperature, humidity, lighting, and other parameters essential for plant growth and development. The chamber's core function is to create a stable and optimized environment for plant research, experimentation, and production.
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Lab products found in correlation
4 protocols using plant growth chamber
1
Plant Growth Conditions for Brassica and Hordeum
2
Arabidopsis thaliana Growth Conditions
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As plant materials in the present study, we used wild-type A. thaliana (Columbia-0 ecotype) and if1 mutant lines (SALK_000139) from the Arabidopsis Information Resource. The transgenic functional complementary lines (p35S:AtIF1-if1) were generated through expression of AtIF1 in if1 mutant line. More details of plant materials were shown in our previous study (Chen et al., 2020). Seeds were surface-sterilized for 4–12 h with Cl2 gas produced by the reaction between 5% (v/v) NaClO and concentrated HCl solution, after which they were seeded on 1/2 Murashige and Skoog medium (Sigma-Aldrich, St. Louis, MO, USA) with 0.8% agar and 1% sucrose. Following stratification treatment at 4 °C for 2–4 days under dark conditions, the plates were transferred to a plant growth chamber (Sanyo, Osaka, Japan) under long-day conditions, 16 h day (120 μmol m−2 s−1, 22 °C) and 8 h night (21 °C) at 55–60% relative humidity. Seedlings were grown for 4 weeks before analysis. Having collected whole plants, the samples were immediately frozen in liquid nitrogen and stored at −80 °C. Seven seedlings were harvested as one biological repeat. In total, 3 independent biological repeats were used for further analysis.
3
Sterilization and Germination of Arabidopsis and Cotton
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Seeds of Arabidopsis thaliana (ecotype Columbia) were surface-sterilized with 75% ethanol for 1 min and 5% NaClO for 3 min, followed by washing with sterile distilled water. Sterilized Arabidopsis seeds were sown on half-strength Murashige-Skoog (MS) media (10 g L 1 sucrose, pH 5.8) maintained at 4°C for 48 h. The plates were then transferred to a plant growth chamber (Sanyo, Osaka, Japan) for 7 d before transplantation into soil (16 h light/8 h dark, 22±1°C).
Cotton (Gossypium hirsutum cv. Coker 312) seeds were surface-sterilized with 75% (v/v) ethanol for 1 min and 10% (v/v) H 2 O 2 for 2 h, followed by washing with sterile distilled water. Sterilized cotton seeds were sown on half-strength MS media (16 h light/8 h dark, 28°C) for 5 d, and then transplanted into soil for growth to maturation.
Cotton (Gossypium hirsutum cv. Coker 312) seeds were surface-sterilized with 75% (v/v) ethanol for 1 min and 10% (v/v) H 2 O 2 for 2 h, followed by washing with sterile distilled water. Sterilized cotton seeds were sown on half-strength MS media (16 h light/8 h dark, 28°C) for 5 d, and then transplanted into soil for growth to maturation.
4
Comparative Clusia Species Growth and Propagation
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A previously characterised glasshouse collection of 11 Clusia species was used for comparative analyses. Plants aged 3-6 years (approx. 60-100 cm tall) were grown in 3:1, (v/v) compost-sand mixture (John Innes No. 2, Sinclair Horticulture Ltd, Lincoln, UK), in 10 L pots.
Plants aged 3-6 years (approx. 60-100 cm tall) were grown in 3:1, (v/v) compost-sand mixture (John Innes No. 2, Sinclair Horticulture Ltd, Lincoln, UK), in 10 L pots. Plants were lit with sunlight plus photosynthetic LED lights (Attis 5 LED plant growth light, PhytoLux) to ensure that a 12 h photoperiod was maintained throughout the year. Plants were watered every two days and glasshouse temperatures were maintained at 25 and 23 C during the day and night, respectively.
Clusia alata (constitutive CAM) and C. tocuchensis (obligate C3) plants were used for leaf dissections (described below). These plants were propagated from cuttings, as described in Barrera-Zambrano et al., (2014) (link). The 3-4-year-old plants were then transferred to a plant growth chamber (SANYO Fitotron) and allowed to acclimatise for 3 weeks (12-hour light period, day/night temp 25/19 °C). Plants received approx. 500 μM m -2 s -1 PFD at leaf height and were watered every two days.
Plants aged 3-6 years (approx. 60-100 cm tall) were grown in 3:1, (v/v) compost-sand mixture (John Innes No. 2, Sinclair Horticulture Ltd, Lincoln, UK), in 10 L pots. Plants were lit with sunlight plus photosynthetic LED lights (Attis 5 LED plant growth light, PhytoLux) to ensure that a 12 h photoperiod was maintained throughout the year. Plants were watered every two days and glasshouse temperatures were maintained at 25 and 23 C during the day and night, respectively.
Clusia alata (constitutive CAM) and C. tocuchensis (obligate C3) plants were used for leaf dissections (described below). These plants were propagated from cuttings, as described in Barrera-Zambrano et al., (2014) (link). The 3-4-year-old plants were then transferred to a plant growth chamber (SANYO Fitotron) and allowed to acclimatise for 3 weeks (12-hour light period, day/night temp 25/19 °C). Plants received approx. 500 μM m -2 s -1 PFD at leaf height and were watered every two days.
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