Western blotting was performed as described previously.21 Anti-MYC monoclonal antibody (Abcam, 1:1000), anti-PCYT1A monoclonal antibody (Cell Signaling, 1:1000), anti-p-AKT monoclonal antibody (Cell Signaling, 1:1000), anti-p-ERK monoclonal antibody (Cell Signaling, 1:1000) and Anti-CDK6 monoclonal antibody (Cell Signaling, 1:1000) were used. Anti-Actin antibody (Cell Signaling, 1:2000) was used to ensure equivalent loading of total protein.
Anti p akt monoclonal antibody
Manufactured by Cell Signaling Technology
The Anti-p-AKT monoclonal antibody is a laboratory research tool designed to detect the phosphorylated form of the AKT protein. AKT is a key signaling molecule involved in various cellular processes. This antibody can be used to measure the activation status of AKT in cell-based assays or western blot experiments.
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Lab products found in correlation
Anti p erk monoclonal antibody, by Cell Signaling Technology (1 mentions)
Anti actin antibody, by Cell Signaling Technology (1 mentions)
Anti myc monoclonal antibody, by Abcam (1 mentions)
Anti c myc monoclonal antibody, by Cell Signaling Technology (1 mentions)
Anti akt monoclonal antibody, by Cell Signaling Technology (1 mentions)
Anti gapdh, by Santa Cruz Biotechnology (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
2 protocols using anti p akt monoclonal antibody
1
Western Blot Analysis of Cellular Proteins
2
Protein Expression Analysis of Cell and Exosome Lysates
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Total protein from cells and exosomes were prepared in RIPA buffer with protease inhibitors and quanti ed using BCA assay kit (Thermo Fisher Scienti c Inc., MA, USA). Subsequently, protein lysates were resolved by SDS-PAGE, transferred onto PVDF membranes (Millipore, Bedford, MA). The antibodies used in the experiments included anti-β-catenin monoclonal antibody (1:1000, Cell Signaling Technology), anti-LEF1 monoclonal antibody (1:1000, Cell Signaling Technology), anti-c-myc monoclonal antibody (1:1000, Cell Signaling Technology), anti-VEGFA monoclonal antibody (1:1000, Abcam), anti-TFEB monoclonal antibody (1:1000, Cell Signaling Technology), anti-PI3K monoclonal antibody (1:1000, Cell Signaling Technology), anti-p-PI3K monoclonal antibody (1:1000, Cell Signaling Technology), anti-AKT monoclonal antibody (1:1000, Cell Signaling Technology), anti-p-AKT monoclonal antibody (1:1000, Cell Signaling Technology), and anti-GAPDH (1:5000, Santa Cruz). Each experiment was repeated three times.
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