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2 protocols using methamphetamine

1

Quantification of Drug Concentrations

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Amphetamine, methAmphetamine, MDMA, MDEA, MDA, morphine, codeine, 6-MAM, cocaine and BE (Lipomed, Weil am Rhein, Germany) were dissolved in a solution of 0.9 % NaCl (B. Braun, Melsungen, Germany) and diluted. The mouth rinse solution in study A had a concentration of 1.0 μg/ml. The incubation drug solution in study B had a concentration of 10 μg/ml.
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2

Acquisition and Characterization of Illicit Drugs

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MDMA, amphetamine, methamphetamine, methcathinone, 4-methylmethcathinone, 4-fluoromethcathione, 4-fluoroamphetamine, 4-fluoroephedrine, ephedrine, MDPBP, MDPPP, MDPV, pyrovalerone, and α-PVP were purchased from Lipomed (Arlesheim, Switzerland). 4-Fluoromethamphetamine, 4-etylmethcathinone, and 4-bromomethcathinone were purchased from Cayman Chemicals (Ann Arbor, MI, USA). Naphyrone was synthesized as previously described (Simmler et al., 2013) . All of the drugs were obtained as racemic hydrochloride salts, with the exception of ephedrine, amphetamine, and methamphetamine, for which the (-)-enantiomer was used. Purity was at least 98% for all of the substances.
Radiochemicals (tritium isotopes) were obtained from Anawa (Wangen, Switzerland) or Perkin Elmer (Schwerzenbach, Switzerland), with the exception of [ 3 H]RO5166017, which was synthesized at Roche (Basel, Switzerland).
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