Leaf anti human mouse rat mr1

Manufactured by BioLegend

Sourced in United States

LEAF™ anti-human/mouse/rat MR1 is a lab equipment product. It is a monoclonal antibody that can be used to detect the MR1 protein in human, mouse, and rat samples.

Automatically generated - may contain errors

Lab products found in correlation

Rat igg2a, by R&D Systems (2 mentions) Vaccinia virus b18r carrier free recombinant protein, by Thermo Fisher Scientific (2 mentions) Rhil 2, by BD (1 mentions) Rhil 18, by R&D Systems (1 mentions) Mouse igg1, by BD (1 mentions) Recombinant human il 2, by BD (1 mentions) Mouse igg1, by R&D Systems (1 mentions) Recombinant human il 18, by R&D Systems (1 mentions)

Spelling variants of this product

Anti-MR1 (3 citations)

2 protocols using leaf anti human mouse rat mr1

Cytokine-Mediated Whole Blood Stimulation

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Cytokines used for whole blood stimulations included: rhIL-2 (BD BioScience), rhIL-12p70 (eBioscience), rhIL-18 (R&D Systems), and rIFN (eBioScience), each at a final concentration of 0.1μg/mL or lower, as determined by cytokine titration experiments. Neutralizing antibodies included rat anti-human IL-2 (clone. MQ1-17H12, BD Biosciences), mouse anti-human IL-12 (clone. 24910. R&D Systems), mouse anti-human IL-18 (clone. 125-2H R&D Systems), LEAF™ anti-human/mouse/rat MR1 (BioLegend, San Diego, CA, USA, catalogue no. 361103), and mouse anti-human TCRα (clone. T10B9.1A-31. BD BioSciences) and the isotype controls, mouse IgG1 (clone. 11711, R&D Systems and Rat IgG2a (clone. 54447, R&D Systems). For type I IFN neutralization, we used vaccinia virus B18R Carrier-Free Recombinant Protein (eBioScience). All neutralizing antibodies were used at a final concentration of 10μg/mL.

Suliman S., Murphy M., Musvosvi M., Gela A., Meermeier E.W., Geldenhuys H., Hopley C., Toefy A., Bilek N., Veldsman A., Hanekom W.A., Johnson J.L., Boom W.H., Obermoser G., Huang H., Hatherill M., Lewinsohn D.M., Nemes E, & Scriba T.J. (2019). MR1-Independent Activation of Human Mucosal-Associated Invariant T Cells by Mycobacteria. The Journal of Immunology Author Choice, 203(11), 2917-2927.

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Optimizing Cytokine-Mediated Whole Blood Stimulations

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Cytokines used for whole blood stimulations included the following: recombinant human IL-2 (BD Biosciences), recombinant human IL-12p70 (eBioscience), recombinant human IL-18 (R&D Systems), and rIFN (eBioScience), each at a final concentration 0.1 μg/ml or lower, as determined by cytokine titration experiments. Neutralizing Abs included rat anti-human IL-2 (clone MQ1-17H12; BD Biosciences), mouse anti-human IL-12 (clone 24910; R&D Systems), mouse anti-human IL-18 (clone 125-2H; R&D Systems), LEAF anti-human/mouse/rat MR1 (catalog no. 361103; BioLegend, San Diego, CA), and mouse anti-human TCRα (clone T10B9.1A-31; BD Biosciences); the isotype controls included mouse IgG1 (clone 11711; R&D Systems) and rat IgG2a (clone 54447; R&D Systems). For type I IFN neutralization, we used vaccinia virus B18R Carrier-Free Recombinant Protein (eBioScience). All neutralizing Abs were used at a final concentration 10 μg/ml.

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