Pharmingen foxp3 staining kit

2‐Cl‐trityl chloride resin (1.1 mmol g⁻¹) was obtained from Nankai University resin Co., Ltd. Fmoc‐amino acids and o‐benzotriazol‐1‐yl‐N,N,N′,N′‐tetramethyluronium hexafluorophosphate (HBTU) were acquired from GL Biochem (Shanghai). Hydogels were prepared and respective endotoxin levels of formula were tested as previously reported.^[23, ^24] Ins2_9‐23 peptide (SHLVEALYLVCGERGR; endotoxins <1 EU mg⁻¹) was supplied by Bankpeptide (Hefei, China). The BD Pharmingen FoxP3 staining kit was from (BD Biosciences, Beijing, China). Aluminum hydroxide was purchased from Sigma‐Aldrich (Shanghai, China). Antibodies used for flow cytometry analysis were purchased from eBioscience (CA, USA) and antibodies used for immunofluorescence staining were obtained from Abcam (Shanghai, China). Cytokine chip microarray was performed by Beijing Genomics Institute (Beijing, China). Recombinant Human Insulin R/CD220 (aa 28‐944) Protein was ordered from R&D Systems (Shanghai, China). Mouse Insulin EZRMI‐13 ELISA kit was from Millipore (Merck, UK). Female NOD/ShiLtJNju mice were supplied by Model Animal Research Center of Nanjing University. All animal procedures were approved by the Centre of Tianjin Animal Experiment Ethics Committee. The accreditation number of the laboratory is SYXK(Jin) 2019‐0003 promulgated by Tianjin Science and Technology Commission.