Ethical approval for this research was confirmed by the scientific and ethics committee of Salahaddin University-Erbil of Iraq. A total of 283 samples from patients with UTI symptoms were screened, and finally, 51 isolates (18.02%) of P. mirabilis were isolated. The specimens were directly inoculated to Tryptone Soy Broth and streaked onto MacConkey agar and Blood agar plates and incubated aerobically at 37 °C for 24 hrs. The isolates were identified based on a series of microbiological and biochemical tests that routinely are used in microbiological diagnostic laboratories and confirmatory VITEK II system using (ID) GN cards (BioMérieux Inc. France). All positive cultures were undergoing the PCR assay for amplifying the ID gene to support the identification of P. mirabilis.
Id gn cards
Manufactured by bioMérieux
Sourced in France
The ID GN cards are a laboratory test product used for the identification of Gram-negative bacteria. The cards contain a panel of dehydrated biochemical substrates that allow for the differentiation and identification of various Gram-negative organisms through a standardized testing process.
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2 protocols using id gn cards
1
Isolation and Identification of Proteus mirabilis in UTI
2
Camel Meat Microbiological Analysis
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Twenty-five grams (25g) of minced camel meat were weighted and suspended into 225 ml of 0.1% sterilized buffered peptone water (Hi Media, India) in a sterile stomacher bags and homogenized by shaking for 5 minutes. For enrichment purposes, the stomacher bags were incubated at 37 °C for 24 hours. A loop-full (10 µl) from the overnight growth was streaked on MacConkey agar (Oxoid, England) and incubated at 37 °C for 24 hours. Permissive identification was performed based on colony morphology and gram staining. Five suspected colonies from each sample were purified on brain heart infusion agar (Oxoid, England) and examined for their oxidase activity by oxidase discs (Hi Media, India). Oxidase negative isolates were subjected to further biochemical identification by VITEK 2 compact system using ID-GN cards (bioMérieux, France).
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