F 25 hd temperature control unit

CD spectra were recorded on a Jasco J-810 spectropolarimeter equipped with a Julabo F-25/HD temperature control unit. Hellma quartz cells (10 mm path length, 3000 ml volume) were used. NMR spectra were acquired either in a Bruker Advance spectrometer operating at 600 MHz (sequences CC, AA, GG, and CG) or in a Bruker Digital Avance 800 MHz (sequences TT, TA, CA and TG). Water suppression was achieved by the inclusion of a WATERGATE 16 module in the pulse sequence prior to acquisition. Absorbance spectra were recorded on an Agilent 8453 diode array spectrophotometer. The temperature was controlled by means of an 89090A Agilent Peltier device. Hellma quartz cells (1 or 10 mm path length, and 350, 1500 or 3000 ml volume) were used. The chromatographic system consisted of an Agilent 1100 Series HPLC instrument equipped with a G1311A quaternary pump, a G1379A degasser, a G1392A autosampler, a G1315B photodiode-array detector furnished with a 13-μL flow cell, and an Agilent Chemstation for data acquisition and analysis (Rev. A 10.02), all from Agilent Technologies (Waldbronn, Germany). A BioSep-SEC-S 3000 column (300 × 7.8 mm, particle size 5 μm and pore size 290 Å) from Phenomenex (Torrance, CA, USA) was used for the chromatographic separation at room temperature.

Absorbance spectra were recorded on an Agilent 8453 diode array spectrophotometer (Agilent Technologies; Waldbronn, Germany). Temperature was controlled by means of an Agilent 89090A Peltier device (Agilent Technologies). CD spectra were recorded on a Jasco J-810 spectropolarimeter equipped with a JULABO F-25-HD temperature control unit (Seelbach, Alemania). Fluorescence spectra were measured with an Aminco-Bowman Series 2 spectrofluorimeter (Thermo-Spectronic, USA), equipped with xenon lamp. Temperature was controlled by means of a water bath. Excitation wavelength was depending on QBA used for titration, ranging from 330 to 350 nm.
Emission wavelength for complex QBA:DNA was set to 600 nm. In all spectroscopic studies, Hellma quartz cells (10 mm path length, and 350, 1500 or 3000 µl volume) were used. The NMR spectra were recorded on a Bruker AV600 spectrometer operating at a frequency of 600.10 MHz, equipped with a 5 mm TXI inverse probe and z -axis gradients. The 1 H spectra were referenced to external DSS (2,2-dimethyl-2-silapentane-5-sulfonate sodium salt) set at 0.00 ppm.