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2 protocols using n dodecyl β d maltopyranoside ddm

1

Purification and Reconstitution of Membrane Proteins

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Chemicals were from the following sources: Isopropyl-β-D-thiogalactoside (IPTG) (AmericanBio), His60 Ni Superflow Resin (Takara), tris (2-carboxyethyl) phosphine (TCEP) (Thermo), Iodixanol solution (60%, w/v) (Cosmo Bio USA), Octyl β-D-glucopyranoside (OG) (EMD Millipore), n-dodecyl-β-D-maltopyranoside (DDM) (Avanti Polar Lipids). All DNA oligonucleotides were purchased from Integrated DNA Technologies. All lipids were obtained from Avanti Polar Lipids: 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC); 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS); L-α-phosphatidylinositol- 4,5-bisphosphate [PI(4,5)P2]; 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2–1,3-benzoxadiazol-4-yl) (NBD-PE); 1,2-dioleoyl-sn-glycero-3-phospho-L-serine-N-(7-nitro-2–1,3-benzoxadiazol-4-yl) (NBD-PS); 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rhod-PE); 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl) iminodiacetic acid) succinyl] [DGS-NTA(Ni)]; 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[4-(p-maleimidophenyl)butyramide] (MPB-PE).
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2

Purification and Reconstitution of Membrane Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Chemicals were from the following sources: Isopropyl-β-D-thiogalactoside (IPTG) (AmericanBio), His60 Ni Superflow Resin (Takara), tris (2-carboxyethyl) phosphine (TCEP) (Thermo), Iodixanol solution (60%, w/v) (Cosmo Bio USA), Octyl β-D-glucopyranoside (OG) (EMD Millipore), n-dodecyl-β-D-maltopyranoside (DDM) (Avanti Polar Lipids). All DNA oligonucleotides were purchased from Integrated DNA Technologies. All lipids were obtained from Avanti Polar Lipids: 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC); 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS); L-α-phosphatidylinositol- 4,5-bisphosphate [PI(4,5)P2]; 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2–1,3-benzoxadiazol-4-yl) (NBD-PE); 1,2-dioleoyl-sn-glycero-3-phospho-L-serine-N-(7-nitro-2–1,3-benzoxadiazol-4-yl) (NBD-PS); 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rhod-PE); 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl) iminodiacetic acid) succinyl] [DGS-NTA(Ni)]; 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-[4-(p-maleimidophenyl)butyramide] (MPB-PE).
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