C20 0

Manufactured by Avanti Polar Lipids

C20:0 is a saturated fatty acid with a chain length of 20 carbon atoms. It is commonly used as a standard reference compound in analytical applications.

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Lab products found in correlation

C22 0, by Avanti Polar Lipids (4 mentions) C24 0, by Avanti Polar Lipids (4 mentions) C18 0, by Avanti Polar Lipids (3 mentions) C16 0, by Avanti Polar Lipids (2 mentions) C24 1, by Avanti Polar Lipids (2 mentions) Bile acid standards, by Steraloids (1 mentions) C17 0, by Avanti Polar Lipids (1 mentions) Hplc ms grade methanol, by Merck Group (1 mentions) Analytical grade chloroform, by Merck Group (1 mentions) Ammonium acetate, by Merck Group (1 mentions) Formic acid, by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) Masslynx v4, by Waters Corporation (1 mentions) Bca protein assay kit, by Thermo Fisher Scientific (1 mentions)

4 protocols using c20 0

Bile Acid and Ceramide Lipid Sources

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Bile acids were ordered from Steraloids, Inc., (Newport, RI) and Sigma-Aldrich (St Louis, MO), and T-β-MCA-d5 sodium salt was from Toronto Research Chemicals, Inc., (Toronto, Ontario). C16:0, C18:0, C20:0, C22:0, C24:0 and C24:1 ceramides were obtained from Avanti Polar Lipids. HFD (60 kcal % fat) were purchased from Bio-Serv (Frenchtown, NJ). Gly-MCA was synthesized as according to the Supplementary Fig. 16.

Jiang C., Xie C., Lv Y., Li J., Krausz K.W., Shi J., Brocker C.N., Desai D., Amin S.G., Bisson W.H., Liu Y., Gavrilova O., Patterson A.D, & Gonzalez F.J. (2015). Intestine-selective farnesoid X receptor inhibition improves obesity-related metabolic dysfunction. Nature Communications, 6, 10166.

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Synthesis and Standards for Lipid Analysis

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Gly‐MCA was synthesized by a previously described method.^[⁸^] Bile acid standards were obtained from Steraloids, Inc. or Sigma‐Aldrich. Ceramide standards (C16:0, C17:0, C18:0, C20:0, C22:0, C24:0, and C24:1) were purchased from Avanti Polar Lipids. Low‐fat diet (LFD; D12450B), Amylin liver NASH model diet (AMLN diet; D09100301), methionine‐choline‐sufficient diet (MCS; A02082003B), and methionine and choline‐deficient diet (MCD; A02082002B) were purchased from Research Diet.

Jiang J., Ma Y., Liu Y., Lu D., Gao X., Krausz K.W., Desai D., Amin S.G., Patterson A.D., Gonzalez F.J, & Xie C. (2022). Glycine‐β‐muricholic acid antagonizes the intestinal farnesoid X receptor–ceramide axis and ameliorates NASH in mice. Hepatology Communications, 6(12), 3363-3378.

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Lipidomic Analysis of Long-Chain Lipids

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The chemical standards of C20:0, C22:0, C24:0, and C26:0‐LPC and isotope labeled internal standard C26:0‐d4‐LPC were purchased from Avanti Polar Lipids, Inc. HPLC‐MS grade methanol and acetonitrile were purchased from Merck. Analytical grade chloroform, formic acid, and ammonium acetate were purchased from Sigma‐Aldrich. Filter paper grade 903 was obtained from Whatman GmbH.

Yue X., Liu W., Liu Y., Shen M., Zhai Y., Ma Z, & Cao Z. (2021). Development, validation, and clinical application of an FIA‐MS/MS method for the quantification of lysophosphatidylcholines in dried blood spots. Journal of Clinical Laboratory Analysis, 36(1), e24099.

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Serum and Intestinal Organoid Lipidomics

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Serum global lipidomics was performed as previously described^{55 (link)}. Briefly, 50 μl of serum was extracted with 200 μl of a chloroform:methanol (2:1) solution. After vortex and centrifuge, the lower organic phase was collected and evaporated. For serum global lipidomics, the multivariate data matrix was analysed by SIMCA-P+ 15 software (Umetrics). For ceramide quantification, the data were analysed by TargetLynx software, a subroutine of the MassLynx v4.2 software (Waters). The ceramide standards, including C16:0, C18:0, C18:1, C20:0, C22:0, C24:0 and C24:1, were obtained from Avanti Polar Lipids.
For quantification of ceramide in intestinal organoids and culture medium, intestinal organoids were homogenized with 250 μl deionized water. A total of 200 μl of the homogenized organoids or 200 μl of the culture medium was extracted with 800 μl of a chloroform:methanol (2:1) solution and then processed the same as for serum lipidomics. The remaining homogenized organoids were used to measure protein concentration by the BCA protein assay kit (Pierce Chemical). Ceramide levels of intestinal organoids and culture medium were normalized to the total protein content of the organoids.

Luo Y., Yang S., Wu X., Takahashi S., Sun L., Cai J., Krausz K.W., Guo X., Dias H.B., Gavrilova O., Xie C., Jiang C., Liu W, & Gonzalez F.J. (2021). Intestinal MYC modulates obesity-related metabolic dysfunction. Nature metabolism, 3(7), 923-939.

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