Dopg 840475c

All the lipids used in the experiments were purchased from Avanti Polar Lipids, namely, DOPC (850375C), DOPG (840475C), porcine brain sphingomyelin (860062C), and Cardiolipin (710335C). For performing pelleting assays with liposomes mimicking S. citri lipid composition, the following protocol (earlier reported in Harne et al., 2020 (link)) was used. A stock concentration of 2 mM lipids in chloroform was made for S. citri membrane mimic, only DOPG, only DOPC, and varying percentage ratios of DOPC:DOPG lipids. Chloroform solution of lipids was aliquoted in a clean test tube and dried. Dried lipids were resuspended in buffer A (50 mM Tris, pH 8, and 300 mM KCl) and 1 mM MgCl₂. This lipid solution was extruded through a 100-nm polycarbonate membrane (Avanti Polar Lipids) to get liposomes of 100-nm range. These liposomes were further used in the charge specificity and nucleotide-dependent liposome pelleting assays.