Ecl detection kit

PrimeScript^TM RT reagent kit with gDNA Eraser (Perfect Real Time) (Code No.: RR047A)and SYBR^® Premix Ex Taq^TM II (Tli RNaseH Plus) (Code No.: RR820A) were purchased from TaKaRa. BCA protein assay kit (KGP902) and ECL detection kit were purchased from KeyGENE (Nanjing, China). Cell culture dishes were purchased from Guangzhou Jet Bio-Filtration Co., Ltd. (Guangzhou, China). Antibodies against β-actin (60008-1-lg, mouse), interleukin (IL)-6 (21865-1-AP, rabbit), tumor necrosis factor alpha (TNF-α; 60291-1-lg, mouse), protein kinase B (AKT; 10176-1-AP, rabbit), phosphoinositide 3-kinase (PI3K; 60225-1-lg, mouse), inducible nitric oxide synthase (iNOS; 18985-1-AP, rabbit), arginase-1 (ARG1; 16001-1-AP, rabbit), SRC (11097-1-AP, rabbit), and IL-10 (20850-1-AP, rabbit) were obtained from Proteintech (Wuhan, China). Antibodies against IL-1β (bs-20448R, rabbit), IL-12 (bs-1789R, rabbit), and P-AKT (bs-0876R, rabbit) were purchased from Bioss (Beijing, China). Antibody against P-PI3K (4228, rabbit) was purchased from CST. Dasatinib inhibitor was obtained from MCE (New Jersey, United States). lipofectamine^TM 3000 Reagent was purchased from Thermo Scientific^TM (United States). Mouse IL-6 ELISA kit was purchased from Boster (Wuhan, China).

A lipid peroxidation MDA assay kit (S0131) and a hydrogen peroxide assay kit (s0038) as well as a total glutathione peroxidase assay kit (s0058) were purchased from Beyotime Biotechnology (Shanghai, China). BCA protein assay kit (KGP902) and ECL detection kit were purchased from KeyGENE (Nanjing, China). PrimeScript™ RT reagent kit with gDNA Eraser (Perfect Real Time) (code number RR047A) and SYBR® Premix Ex Taq™ II (Tli RNaseH Plus) (code number RR820A) were purchased from TaKaRa. β-Actin monoclonal antibody was purchased from Zhongshan Jinqiao (Beijing, China). Rabbit polyclonal NOX4 antibody (14347-1-AP) and rabbit polyclonal AGTR1 antibody (25343-1-AP) were purchased from Proteintech (Wuhan, China). NF-κB-p65 antibody and NF-κB-pp65 (ser536) antibody were obtained from Wanleibio (Shenyang, China). Rabbit polyclonal TNF-α antibody was purchased from Bioworld (Nanjing, China). Rabbit polyclonal ACE antibody was purchased from ABclonal (Wuhan, China).