Digital ft nmr spectrometer

All chemicals were purchased either from Sigma-Aldrich (Sigma-Aldrich Corp., St. Louis, MO, USA) or Merck (Merck KGaA, Darmstadt, Germany) and used without further chemical purification. Melting points of the synthesized compounds were determined by a MP90 digital melting point apparatus (Mettler Toledo, Columbus, OH, USA) and were uncorrected. ¹H- and ¹³C-NMR spectra were recorded in DMSO-d₆ by a Bruker digital FT-NMR spectrometer (Bruker Bioscience, Billerica, MA, USA) at 300/75 MHz. The IR spectra were obtained on an IR Prestige-21 spectrophotometer (Shimadzu, Tokyo, Japan). LC-MS-MS studies were performed on a Shimadzu IT-TOF-LC-MS-MS system. The purities of compounds were checked by TLC on silica gel 60 F254 (Merck KGaA).

The complete chemicals used in the synthetic process were acquired
from Merck Chemicals (Merck KGaA, Darmstadt, Germany) or Sigma-Aldrich
Chemicals (Sigma-Aldrich Corp., St. Louis, MO, USA). The compounds’
uncorrected melting points were ascertained using an MP90 digital
melting point instrument (Mettler Toledo, OH, USA). A Bruker digital
FT-NMR spectrometer (Bruker Bioscience, Billerica, MA, USA) operating
at 300 and 75 MHz registered the¹H- and¹³C NMR
spectra of the compounds produced in DMSO-d₆, respectively. In the NMR spectra, splitting patterns were denoted
as follows: s for singlet, d for doublet, t for triplet, and m for
multiplet. The reported unit of coupling constants (J) was Hertz.
The Shimadzu LC/MSMS system (Shimadzu, Tokyo, Japan) was used to determine
the M + 1 peaks. Using Silica Gel 60 F254 TLC for thin-layer chromatography
(TLC), all reactions were observed.

All of the chemicals used
in the synthetic process were bought from Merck (Darmstadt, Germany)
or Sigma-Aldrich (St. Louis, MO, USA). The MP90 digital melting point
instrument (Mettler Toledo, OH, USA) was used to determine the uncorrected
melting points of the compounds that were obtained. A Bruker digital
FT NMR spectrometer (Bruker Bioscience, Billerica, MA, USA) was used
to record the ¹H and ¹³C NMR spectra of the
produced compounds in DMSO-d₆. Splitting
patterns in the NMR spectra were designated as follows: s, singlet;
d, doublet; t, triplet; m, multiplet. Coupling constants (J) are reported in hertz. [M + 1] peaks were determined
using a Shimadzu LC/MSMS system (Shimadzu, Tokyo, Japan). Using silica
gel 60 F₂₅₄ TLC plates (Merck), thin-layer chromatography
(TLC) was used to monitor each reaction.

All chemicals were obtained either from Sigma-Aldrich Corp. (St. Louis, MO, USA) or Merck KGaA (Darmstadt, Germany), and used without further chemical purification. Melting points of the compounds were measured by using an automatic melting point determination instrument (MP90, Mettler-Toledo, Columbus, OH, USA) and were presented as uncorrected. ¹H- and ¹³C-NMR spectra were recorded in DMSO-d₆ by a Bruker digital FT-NMR spectrometer (Bruker Bioscience, Billerica, MA, USA) at 300 MHz and 75 MHz, respectively. The IR spectra of the compounds were recorded using an IRAffinity-1S Fourier transform IR (FTIR) spectrometer (Shimadzu, Tokyo, Japan). MS studies were performed on an LCMS-8040 tandem mass system (Shimadzu, Tokyo, Japan). Chemical purities of the compounds were checked by classical TLC applications performed on silica gel 60 F254 (Merck KGaA).

All reagents were purchased from commercial suppliers and were used without further purification. Melting points (M.p.) were determined on an Electrothermal 9100 melting point apparatus (Weiss-Gallenkamp, Loughborough, UK) and are uncorrected. IR spectra were recorded on an IRPrestige-21 Fourier Transform Infrared spectrophotometer (Shimadzu, Tokyo, Japan). ¹H NMR and ¹³C NMR spectra were recorded by a Bruker digital FT-NMR spectrometer (Bruker Bioscience, Billerica, MA, USA) in DMSO-d₆. Mass spectra were recorded on an Agilent LC-MSD-Trap-SL Mass spectrometer (Agilent Technologies, Palo Alto, CA, USA). Elemental analyses were performed on a Perkin Elmer EAL 240 elemental analyzer (Perkin-Elmer, Norwalk, CT, USA) and the results were within ±0.4% of the theoretical values. Thin Layer Chromatography (TLC) was performed on TLC Silica gel 60 F254 aluminium sheets (Merck, Darmstadt, Germany) to check the purity of the compounds.

All chemicals were obtained either from Sigma-Aldrich (Sigma-Aldrich Corp., St. Louis, MO, USA) or Merck (Merck KGaA, Darmstadt, Germany), and used without further purification. Melting points of the compounds were measured by using an automatic melting point determination instrument (MP90, Mettler-Toledo, Columbus, OH, USA) and are uncorrected. ¹H- and ¹³C-NMR spectra were recorded in DMSO-d₆ on a Bruker digital FT-NMR spectrometer (Bruker Bioscience, Billerica, MA, USA) at 300 MHz and 75 MHz, respectively. The IR spectra of the compounds were recorded using an IRAffinity-1S Fourier transform IR (FTIR) spectrometer (Shimadzu, Tokyo, Japan). HRMS studies were performed on an LCMS-IT-TOF system (Shimadzu). Chemical purities of the compounds were checked by classical TLC applications performed on silica gel 60 F254 (Merck KGaA).