Human oesophageal cancer cell lines Kyse150, Kyse170, TE1 and Eca109 were purchased from American Type Culture Collection and were cultured in RPMI 1640 (Invitrogen) medium containing 10% foetal bovine serum (Invitrogen) at 37°C in an atmosphere containing 5% CO2. The cells were treated with 10 ng/mL of recombinant TGF‐β1 (R&D Systems) for 7 days with the medium replenishment every 2 days.
Kyse170
Manufactured by American Type Culture Collection
The Kyse170 is a piece of laboratory equipment designed for cell culture applications. It is a cell line derived from human esophageal squamous cell carcinoma. The Kyse170 cell line is intended for use in research and experimental settings, where it can serve as a model system for the study of esophageal cancer.
Automatically generated - may contain errors
Lab products found in correlation
Kyse 150, by American Type Culture Collection (3 mentions)
Eca109, by American Type Culture Collection (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Recombinant tgf β1, by R&D Systems (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Hek293t, by American Type Culture Collection (1 mentions)
Ht1080, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Hek293t, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Ht1080, by American Type Culture Collection (1 mentions)
Kyse170, by Thermo Fisher Scientific (1 mentions)
Kyse 150, by Merck Group (1 mentions)
4 protocols using kyse170
1
TGF-β1 Treatment of Esophageal Cancer Cells
2
TGF-β1 Induces SNHG17 Expression
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Human ESCC cell lines (TE1, Eca109, Kyse150, Kyse170, and Yes2) were purchased from the American Type Culture Collection and maintained in RPMI‐1640 medium (Invitrogen) supplemented with 10% fetal bovine serum (Invitrogen). When cells reached 70%‐80% confluency, cells were stimulated with 10 ng/mL recombinant TGF‐β1 (R&D Systems Inc) for 7 d. Then, total RNA was extracted and reverse transcribed into cDNA to examine SNHG17 expression.
3
Establishing Stable Cell Lines for Protein Expression
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HEK293T, HT-1080 and KYSE-170 cells were purchased from the American Type Culture Collection (ATCC). HEK293T and HT-1080 cells were cultured in DMEM (Invitrogen) supplemented with 5% FBS (Gibco), 100 unit/mL penicillin, and 100 mg/mL streptomycin (Gibco). KYSE-170 cells were cultured in RPMI 1640 medium (Gibco) with 10% FBS, 100 unit/mL penicillin, and 100 mg/mL streptomycin.
Cell transfection was carried out by Lipofectamine 2000 according to the manufacturer’s protocol (Invitrogen).
Cells stably expressing the indicated proteins were established by standard retroviral infection, and selected in 2 mg/mL puromycin (Ameresco) or 50 mg/mL hygromycin B (Ameresco) for 7 days. The mutant IDH1 allele knocked out HT-1080(IDH1+/−) cells were generated previously by TALEN technology.
Cell transfection was carried out by Lipofectamine 2000 according to the manufacturer’s protocol (Invitrogen).
Cells stably expressing the indicated proteins were established by standard retroviral infection, and selected in 2 mg/mL puromycin (Ameresco) or 50 mg/mL hygromycin B (Ameresco) for 7 days. The mutant IDH1 allele knocked out HT-1080(IDH1+/−) cells were generated previously by TALEN technology.
4
Esophageal Cancer Cell Line Cultivation
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Human esophageal cancer cell lines (Kyse150, Kyse170, Eca109, and TE1) were purchased from American Type Culture Collection (ATCC). Cells were cultured in RPMI-1640 (Invitrogen, Carlsbad, CA, USA) containing 10% heat-inactivated fetal bovine serum (Invitrogen) in an atmosphere containing 5% CO2 at 37 °C. For Trichostatin A (TSA) (CAS No. 58880-19-6, Sigma) treatment, Kyse 150 and Kyse170 cells were transfected for 12–24 h, then treated with 300 nM TSA for additional 48 h.
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