The strains Tannerella forsythia ATCC 43037 and Prevotella intermedia ATCC 25611 were acquired from commercial providers (American Type Culture Collection, Manassas, USA), and cultivated on COL agar plates (BD, Franklin Lakes, USA) and in PYG modified with 5 μg/ml hemin and 1% vitamin K1 under anaerobic atmosphere (37 °C, 80% N2/10% H2/10% CO2). Growth behavior in cell culture medium DMEM with 10% FCS was validated via optical density at 600 nm in parallel with PYG and BHI and parallel CFU determination of BHI probes on BHI agar plates over 24 h under anaerobic conditions.
Prevotella intermedia atcc 25611
Manufactured by American Type Culture Collection
Sourced in United States
Prevotella intermedia ATCC 25611 is a laboratory strain of the anaerobic bacterium Prevotella intermedia. It is available for purchase from the American Type Culture Collection (ATCC) as a frozen culture.
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2 protocols using prevotella intermedia atcc 25611
1
Cultivation and Growth Behavior of Anaerobic Oral Bacteria
2
Culturing Prevotella Anaerobic Bacteria
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Prevotella. intermedia (ATCC 25611) and P. gingivalis (ATCC 33277) cultures were obtained from the American Type Culture Collection (ATCC, USA). Microorganisms were cultured on Brain Heart Infusion Agar (BHIA) supplemented with 5% sheep blood (Salubris, Turkey) and incubated in an anaerobic workstation (Don Whitley, UK) at 37°C, in an atmosphere of 80% N2, 10% H2, and 10% CO2 (HABAS, Turkey) for 3 - 4 d. Cultures were then maintained by sub-culturing every 2 - 3 d.
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